Cancers, Vol. 12, Pages 1912: Role of ETS1 in the Transcriptional Network of Diffuse Large B Cell Lymphoma of the Activated B Cell-Like Type

Cancers, Vol. 12, Pages 1912: Role of ETS1 in the Transcriptional Network of Diffuse Large B Cell Lymphoma of the Activated B Cell-Like Type Cancers doi: 10.3390/cancers12071912 Authors: Valdemar Priebe Giulio Sartori Sara Napoli Elaine Yee Lin Chung Luciano Cascione Ivo Kwee Alberto Jesus Arribas Afua Adjeiwaa Mensah Andrea Rinaldi Maurilio Ponzoni Emanuele Zucca Davide Rossi Dimitar Efremov Georg Lenz Margot Thome Francesco Bertoni Diffuse large B cell lymphoma (DLBCL) is a heterogenous disease that has been distinguished into at least two major molecular entities, the germinal center-like B cell (GCB) DLBCL and activated-like B cell (ABC) DLBCL, based on transcriptome expression profiling. A recurrent ch11q24.3 gain is observed in roughly a fourth of DLBCL cases resulting in the overexpression of two ETS transcription factor family members, ETS1 and FLI1. Here, we knocked down ETS1 expression by siRNA and analyzed expression changes integrating them with ChIP-seq data to identify genes directly regulated by ETS1. ETS1 silencing affected expression of genes involved in B cell signaling activation, B cell differentiation, cell cycle, and immune processes. Integration of RNA-Seq (RNA sequencing) data and ChIP-Seq (chromatin immunoprecipitation sequencing) identified 97 genes as bona fide, positively regulated direct targets of ETS1 in ABC-DLBCL. Among these was the Fc receptor for IgM, FCMR (also known as FAIM3 or Toso), which showed high...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research