Cell Monolayer Translocation Assay.

Cell Monolayer Translocation Assay.
Methods Mol Biol. 2020;2134:161-170
Authors: Wunder EA
Abstract
An essential property associated with leptospiral virulence is the pathogen's ability to translocate across host cells, enabling Leptospira to evade the host immune response, disseminate, and establish infection. Cell monolayer translocation assay allows for the quantification of Leptospira strain's competence to cross cell barriers while measuring the integrity of the polarized eukaryotic cell monolayer during this process.
PMID: 32632868 [PubMed - in process]

Source: Mol Biol Cell - July 8, 2020 Category: Molecular Biology Authors: Wunder EA Tags: Methods Mol Biol Source Type: research