Papain immobilized on alginate membrane for wound dressing application.

Papain immobilized on alginate membrane for wound dressing application.
Colloids Surf B Biointerfaces. 2020 Jun 25;194:111222
Authors: Moreira Filho RNF, Vasconcelos NF, Andrade FK, Rosa MF, Vieira RS
Abstract
Wound dressings based on natural polymers are of considerable interest in the pharmaceutical industry owing to their improved performance in the human body when compared to synthetic polymers. Alginate, a polysaccharide from brown algae, is commonly studied as a wound dressing owing to its biocompatibility and biodegradability. To improve its therapeutic features and thereby increase wound healing, papain (a proteolytic enzyme from Carica papaya latex) was proposed to be incorporated. Papain is capable of promoting the debridement of devitalized or necrotic tissues. The development of dressing based on alginate and papain aggregates the healing properties of both materials. In addition, the adsorption on a support can stabilize the enzyme structure and permits its release in a controlled manner. The optimal conditions for immobilization were evaluated (initial concentration, temperature, and pH), and the amount immobilized was measured by Bradford assay. The enzyme activity stability over 28 days was measured. The release profile was determined using Franz cell. In vitro cytotoxicity assays were performed using fibroblasts and keratinocytes. Optimal immobilization conditions were identified in a neutral medium at a papain conce...

Source: Colloids and Surfaces - June 24, 2020 Category: Biotechnology Authors: Moreira Filho RNF, Vasconcelos NF, Andrade FK, Rosa MF, Vieira RS Tags: Colloids Surf B Biointerfaces Source Type: research