18. Two distinct cell lines in one hepatoblastoma tissue block

The patient was a 4 year old male diagnosed with hepatoblastoma. DNA was extracted from paraffin embedded tumor sections and analyzed using the OncoScan CNV assay. Tissue from the same block was independently analyzed on the a targeted DNA hybrid capture NGS platform. Initial microarray results indicated a copy number gain at 1q32.1 (PIK3C2B, MDM4), copy number gains on Chromosome 20 and multiple regions of loss of heterozygosity. In contrast, data from the NGS assay indicated whole chromosome gains of 2, 5, 10 and 17 along with the copy number gain at 1q32.1 (PIK3C2B, MDM4).

Source: Cancer Genetics and Cytogenetics - May 31, 2020 Category: Genetics & Stem Cells Authors: Leslie Grimmett, Joanna Plunkitt, Tamara Restrepo, Alanna Church, Sanda Alexandrescu, Marian Harris Source Type: research