One-Step duplex droplet digital PCR for WT1 overexpression

With the improvement of treatment methods in acute hematology malignancies, the development of sensitive tools for minimal residual disease (MRD) assessment has become a priority. The monitoring of WT1 expression level by Quantitative Polymerase Chain Reaction has been a standard for MRD evaluation in Acute Myeloid Leukemia (AML) and, since 2009, has been optimized through a European LeukemiaNet (ELN) effort in an established protocol with well-defined clinical endpoints. Building on the work of the ELN, we report the development of a novel, One-Step duplex WT1/ABL1 droplet digital assay for WT1 overexpression detection.

Source: Journal of Molecular Diagnostics - June 10, 2020 Category: Pathology Authors: Laurent Dewispelaere, Leonore Bleret, Tom Van Acker, Cindy Van Branteghem, Pascale Cochaux, Pierre Heimann, Hakim El Housni Tags: Technical Advance Source Type: research