Effects of seawater immersion on open traumatic brain injury in rabbit model.

Effects of seawater immersion on open traumatic brain injury in rabbit model. Brain Res. 2020 May 20;:146903 Authors: Liu KL, Yu XJ, Sun TZ, Wang YC, Chen MX, Su YW, Zhang HC, Chen YM, Gao HL, Shi XL, Qi J, Li Y, Li HB, Dong WJ, He JK, Kang YM Abstract We emulated instances of open traumatic brain injuries (TBI) happened in a maritime disaster. New Zealand rabbit animal models were used to evaluate the pathophysiological changes in open TBI with and without the influence of artificial seawater. New Zealand rabbits were randomly divided into 3 groups. Control group consisted of only normal animals. Animals in TBI and TBI+Seawater groups underwent craniotomy with dura mater incised and brain tissue exposed to free-fall impact. Afterward, only TBI+Seawater group received on-site artificial seawater infusion. Brain water content (BWC) and permeability of blood-brain barrier (BBB) were assessed. Reactive oxygen species levels were measured. Western blotting, immunofluorescence were employed to detect: apoptosis-related factors Caspase-3, Bax and Bcl-2; angiogenesis-related factors CD31 and CD34; astrogliosis-related factor glial fibrillary acidic protein (GFAP); potential neuron injury indicator neuron-specific enolase (NSE). Hematoxylin & eosin, Masson-trichrome and Nissl stainings were performed for pathological observations. Comparing to Control group, TBI caused abnormal neurons shape; increased BWC; compromised BBB integrity; inc...
Source: Brain Research - Category: Neurology Authors: Tags: Brain Res Source Type: research