Biochemical and Computational Approaches for the Large-Scale Analysis of Protein Arginine Methylation by Mass Spectrometry.

Biochemical and Computational Approaches for the Large-Scale Analysis of Protein Arginine Methylation by Mass Spectrometry.
Curr Protein Pept Sci. 2020 Apr 26;:
Authors: Musiani D, Massignani E, Cuomo A, Yadav A, Bonaldi T
Abstract
The absence of efficient mass spectrometry-based approaches for the large-scale analysis of protein arginine methylation has hindered the understanding of its biological role, beyond the transcriptional regulation occurring through histone modification. In the last decade, however, several technological advances of both the biochemical methods for methylated polypeptide enrichment and the computational pipelines for MS data analysis have considerably boosted this research field, generating novel insights about the extent and role of this post translational modification. Here, we offer an overview on state-of-the-art approaches for the high-confidence identification and accurate quantification of protein arginine methylation by high-resolution mass spectrometry methods, which comprise the development of both biochemical and bioinformatics methods. The further optimization and systematic application of these analytical solutions will lead to groundbreaking discoveries on the role of protein methylation in biological processes.
PMID: 32338214 [PubMed - as supplied by publisher]

Source: Current Protein and Peptide Science - April 25, 2020 Category: Biochemistry Authors: Musiani D, Massignani E, Cuomo A, Yadav A, Bonaldi T Tags: Curr Protein Pept Sci Source Type: research