Agreement of capillary-obtained acylated ghrelin, active glp-1, glucagon, insulin and leptin with their venous equivalents.

Agreement of capillary-obtained acylated ghrelin, active glp-1, glucagon, insulin and leptin with their venous equivalents. Br J Sports Med. 2013 Nov;47(17):e4 Authors: Green B, Gonzalez J, Thomas K, Dodd-Reynolds C, Bryans J, Stevenson E, Rumbold P Abstract The purpose of the present study was to examine whether the expression of capillary-derived appetite peptides accurately reflects concentrations of their venous equivalents. Fingertip capillary (2×0.3 mL) and venous blood samples (2×4 mL) were collected simultaneously from 19 healthy adult volunteers [mean age 24.1 (SD 5.7) years, body mass 73.7 (SD 10.9) kg;], for determination of acylated ghrelin, GLP-17-36, glucagon, insulin and leptin. Samples were obtained at baseline (t=0 min) and at 30, 60, 90 and 120 min following consumption of a standardised breakfast. Bland-Altman plots were constructed with their 95% limits of agreement and confidence intervals. All analysis was computed using time-averaged area under the curve data (AUC). Where data were heteroscedastic, data were log transformed and reported as ratio limits of agreement (LOA). With the exception of acylated ghrelin (r(2)=0.51), venous and capillary blood samples displayed strong correlations between all hormonal peptides (r(2)≥0.84). GLP-17-36 and glucagon illustrated no systematic difference between capillary and venous blood samples (0.44±0.63 and -0.19±7.63 pg·mL(-1), respectively). Capillary leptin, acylated ghreli...
Source: British Journal of Sports Medicine - Category: Sports Medicine Authors: Tags: Br J Sports Med Source Type: research