Teratozoospermia: its association with sperm DNA defects, apoptotic alterations and oxidative stress

This study carried out to evaluate the level of nuclear sperm DNA damage in men with isolated poymorphic  teratozoospermia and examining its relationship with apoptosis and oxidative stress.A total of 89 subjects was divided into two groups, men with isolated teratozoospermia (n = 69) and men with normal semen parameters (n = 20) as controls. Sperm DNA breaks were determined by using acridine orange staining. The proportion of viable spermatozoa with mitochondrial transmembrane depolarization was detected by fluorescence microscopy through the use of MitoPT ‐JC‐1 staining method. Bivariate Annexin V/ 6‐CFDA analysis was then set out in order to measure the percentage of both viable and dead spermatozoa with phosphatidylserine (PS) externalization. Seminal antioxidant profile (reduced Glutathione (GSHr); Oxidized Glutathione (GSSG); Glutathione‐ S‐transferase (GST)), and total protein sulfhydryl (P‐SH) concentrations were measured spectrophotometrically. Men with isolated teratozoospermia, when compared to controls, showed significantly increased level of single sperm DNA breaks, and higher proportions of spermatozoa with phosphatidylse rine externalization and mitochondrial depolarisation. Among the different studied oxidative stress seminal parameters, the rates of seminal GSHr, GST and P‐SH were significantly decreased in the teratozoospermic group. However, the seminal rates of GSSG and GST have decreased, but only GST didn’ t showed a significant differe...
Source: Andrology - Category: Urology & Nephrology Authors: Tags: ORIGINAL ARTICLE Source Type: research