Cancers, Vol. 12, Pages 537: Elevated Tumor Lactate and Efflux in High-grade Prostate Cancer demonstrated by Hyperpolarized 13C Magnetic Resonance Spectroscopy of Prostate Tissue Slice Cultures

Cancers, Vol. 12, Pages 537: Elevated Tumor Lactate and Efflux in High-grade Prostate Cancer demonstrated by Hyperpolarized 13C Magnetic Resonance Spectroscopy of Prostate Tissue Slice Cultures Cancers doi: 10.3390/cancers12030537 Authors: Sriram Van Criekinge DeLos Santos Ahamed Qin Nolley Santos Tabatabai Bok Keshari Vigneron Peehl Kurhanewicz Non-invasive assessment of the biological aggressiveness of prostate cancer (PCa) is needed for men with localized disease. Hyperpolarized (HP) 13C magnetic resonance (MR) spectroscopy is a powerful approach to image metabolism, specifically the conversion of HP [1-13C]pyruvate to [1-13C]lactate, catalyzed by lactate dehydrogenase (LDH). Significant increase in tumor lactate was measured in high-grade PCa relative to benign and low-grade cancer, suggesting that HP 13C MR could distinguish low-risk (Gleason score ≤3 + 4) from high-risk (Gleason score ≥4 + 3) PCa. To test this and the ability of HP 13C MR to detect these metabolic changes, we cultured prostate tissues in an MR-compatible bioreactor under continuous perfusion. 31P spectra demonstrated good viability and dynamic HP 13C-pyruvate MR demonstrated that high-grade PCa had significantly increased lactate efflux compared to low-grade PCa and benign prostate tissue. These metabolic differences are attributed to significantly increased LDHA expression and LDH activity, as well as significantly increased monocarboxylate tra...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research

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Publication date: Available online 27 March 2020Source: Academic RadiologyAuthor(s): Harrison Kim, John V. Thomas, Jeffrey W. Nix, Jennifer B. Gordetsky, Yufeng Li, Soroush Rais-Bahrami
Source: Academic Radiology - Category: Radiology Source Type: research
Conclusion: future studies with larger populations are needed to explore the nursing diagnoses and effects of nursing interventions on patients who underwent radical prostatectomy.Resumo Objetivo: Determinar os diagn ósticos de enfermagem da NANDA-I e as intervenções de enfermagem (NIC) em pacientes que passaram por prostatectomia radical. Método: um estudo transversal e descritivo foi realizado em um hospital universitário e de pesquisa na Turquia ocidental entre junho de 2016 e junho de 2017. A amostra incluiu pacientes adultos diagnosticados com câncer de próstata no per&i...
Source: Revista da Escola de Enfermagem da USP - Category: Nursing Source Type: research
In this study, we intravenously administrated the young mitochondria into aged mice to evaluate whether energy production increase in aged tissues or age-related behaviors improved after the mitochondrial transplantation. The results showed that heterozygous mitochondrial DNA of both aged and young mouse coexisted in tissues of aged mice after mitochondrial administration, and meanwhile, ATP content in tissues increased while reactive oxygen species (ROS) level reduced. Besides, the mitotherapy significantly improved cognitive and motor performance of aged mice. Our study, at the first report in aged animals, not only prov...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
In this study, the feasibility of the quantification of prostate cancer aggressiveness using parametric methods assessing [11C]-acetate kinetics was investigated in prostate cancer subjects. The underlying uptake mechanism correlated with [11C]-acetate influx and efflux measured in real-time in vitro in cell culture. Methods: Twenty-one patients with newly diagnosed low-to-moderate risk prostate cancer underwent magnetic resonance imaging (MRI) and dynamic [11C]-acetate PET/CT examinations of the pelvis. Parametric images of K1 (extraction × perfusion), k2 (oxidative metabolism) and VT (=K1/k2, anabolic metabolism de...
Source: International Journal of Medical Sciences - Category: Biomedical Science Tags: Int J Med Sci Source Type: research
In conclusion, this study demonstrates the concept of combining docetaxel with the biodegradable microparticles at the point of care is technically feasible for achieving an effective drug-device combination tissue scaffold. This approach could provide an effective new approach for delivering adjuvant chemotherapy following radical prostatectomy. PMID: 31735095 [PubMed - in process]
Source: Drug Delivery - Category: Drugs & Pharmacology Tags: Drug Deliv Source Type: research
ConclusionsIn this paper, technical feasibility of multiparametric machine learning to improve upon single US modalities for the localization of PCa has been demonstrated. Extended datasets for training and testing may establish the clinical value of automatic multiparametric US classification in the early diagnosis of PCa.Key Points• Combination of B-mode ultrasound, shear-wave elastography, and contrast ultrasound radiomics through machine learning is technically feasible.• Multiparametric ultrasound demonstrated a higher prostate cancer localization ability than single ultrasound modalities.• Computer-aid...
Source: European Radiology - Category: Radiology Source Type: research
Abstract13N-Ammonia (13N-NH3) is widely used positron emission tomography/computed tomography (PET/CT) radiotracer for the measurement of myocardial blood perfusion; the possible role of13N-NH3 PET or PET/CT in oncological disease is not yet clear. Aim of this review is to evaluate the diagnostic performances of13N-NH3 PET in this field. A comprehensive computer literature search of the PubMed/MEDLINE, Scopus, and Embase databases was conducted including articles up to June 2019. Eighteen articles were finally included in the review. From the analyses of the selected studies, the following main findings could be drawn: (1)...
Source: Japanese Journal of Radiology - Category: Radiology Source Type: research
To establish the diagnostic performance of the parameters obtained from dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) and diffusion-weighted imaging at 3T in discriminating between non-clinically significant prostate cancers (ncsPCa, Gleason score [GS]  
Source: Canadian Association of Radiologists Journal - Category: Radiology Authors: Tags: Abdominal Imaging / Imagerie abdominale Source Type: research
ConclusionsOur data demonstrate a promising new strategy to predict PPI through the perfusion quality of pelvic muscle structures with contrast media kinetics. This may facilitate preoperative patient consulting and decision-making.
Source: World Journal of Urology - Category: Urology & Nephrology Source Type: research
ConclusionBoth the IVIM and DKI models are beneficial to predict GS of PCa and indirectly predict its aggressiveness, and they have a comparable diagnostic performance with each other as well as ADC.
Source: Abdominal Imaging - Category: Radiology Source Type: research
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