Extracellular vesicles restrict dengue virus fusion in Aedes aegypti cells.

Extracellular vesicles restrict dengue virus fusion in Aedes aegypti cells.
Virology. 2020 Feb;541:141-149
Authors: Freitas MN, Marten AD, Moore GA, Tree MO, McBrayer SP, Conway MJ
Abstract
Aedes aegypti is the primary vector of dengue virus (DENV), and acquires this virus from a vertebrate host during blood feeding. Previous literature has shown that vertebrate blood factors such as complement protein C5a and low-density lipoprotein (LDL) influence DENV acquisition in the mosquito. Here, we show that extracellular vesicles in cell culture medium inhibit DENV infection in mosquito cells. Specifically, extracellular vesicles enter into mosquito cells and inhibit an early stage of infection. Extracellular vesicles had no effect on virus cell attachment or entry. Instead, extracellular vesicles restricted virus membrane fusion. Extracellular vesicles only inhibited DENV infection in mosquito cells and not vertebrate cells. These data highlight a novel virus-vector-host interaction that limits virus infection in mosquito cells by restricting virus membrane fusion.
PMID: 32056712 [PubMed - in process]

Source: Virology - January 31, 2020 Category: Virology Authors: Freitas MN, Marten AD, Moore GA, Tree MO, McBrayer SP, Conway MJ Tags: Virology Source Type: research