DNA methylation-mediated Siglec-7 regulation in natural killer cells via two 5' promoter CpG sites.

In this study, we aimed to investigate how cell surface Siglec-7 expression is regulated and found that, in both NK cell lines and peripheral NK cells, transcription was the main regulatory step. Furthermore, when NK-92MI and peripheral NK cells were treated with DNA methyltransferase (DNMT) inhibitor, the CpG island, with 9 CpG sites, in 5' Siglec-7 promoter became noticeably hypomethylated, and Siglec-7 expression increased in both RNA transcript and surface protein. Within this CpG island, we identified both CpG 8 and CpG 9 as two key regulators responsible for Siglec-7 expression. Additionally, by using histone deacetylases (HDAC) inhibitor, butyric acid, we showed that Siglec-7 expression was also subjected to the histone modification. And a combined treatment with both 5-azacytidine and butyric acid showed an additive effect on Siglec-7 transcript expression in peripheral NK cells.
PMID: 32027025 [PubMed - as supplied by publisher]

Source: Immunology - February 5, 2020 Category: Allergy & Immunology Authors: Huang HT, Su SC, Chiou TJ, Lin YH, Shih YC, Wu YX, Fan TH, Twu YC Tags: Immunology Source Type: research