Defining endogenous barcoding sites for CRISPR/Cas9-based cell lineage tracing in zebrafish

In this study, we characterized 78 high-quality endogenous sites in the zebrafish genome that can be used as CRISPR/Cas9-based barcoding sites. The 78 sites are all highly expressed in most of the cell types according to single-cell RNA sequencing (scRNA-seq) data. Hence, the barcoding information of the 78 endogenous sites is recovered by the available scRNA-seq platforms, enabling simultaneous characterization of cell type and cell lineage information.

Source: Journal of Genetics and Genomics - February 7, 2020 Category: Genetics & Stem Cells Source Type: research