Glycolysis regulates gene expression by promoting the crosstalk between H3K4me3 and H3K14ac in Saccharomyces cerevisiae
Publication date: Available online 11 December 2019Source: Journal of Genetics and GenomicsAuthor(s): Yinsheng Wu, Shihao Zhang, Xuanyunjing Gong, Qi Yu, Yuan Zhang, Mingdan Luo, Xianhua Zhang, Jerry L. Workman, Xilan Yu, Shanshan LiAbstractCells need to coordinate gene expression with their metabolic states to maintain cell homeostasis and growth. However, how cells transduce nutrient availability to appropriate gene expression response via histone modifications remains largely unknown. Here, we report that glucose specifically induces histone H3K4 trimethylation (H3K4me3), an evolutionarily conserved histone covalent mod...
Source: Journal of Genetics and Genomics - December 11, 2019 Category: Genetics & Stem Cells Source Type: research

Drosophila CTP synthase can form distinct substrate- and product-bound filaments
In this study, using negative staining and cryo-electron microscopy, we demonstrate that Drosophila CTPs, whether in substrate-bound or product-bound form, can form filaments. Our results challenge the previous model and indicate that substrate-bound and product-bound filaments can co-exist in the same species. We speculate that the ability to switch between active and inactive cytoophidia in the same cells provides an additional layer of metabolic regulation. (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - December 1, 2019 Category: Genetics & Stem Cells Source Type: research

Site-directed RNA editing (SDRE): Off-target effects and their countermeasures
Publication date: Available online 27 November 2019Source: Journal of Genetics and GenomicsAuthor(s): Shaoshuai Mao, Yajing Liu, Shisheng Huang, Xingxu Huang, Tian ChiAbstractSite-directed RNA editing (SDRE) is invaluable to basic research and medicine, and has emerged as a new frontier in genome editing. The past few years have witnessed a surge of interest in SDRE, with SDRE tools emerging at a breathtaking pace. However, off-target effects of SDRE remain a tough problem, which constitutes a major hurdle to their clinical applications. Here we discuss the diverse strategies for combating off-target editing, drawing lesso...
Source: Journal of Genetics and Genomics - November 28, 2019 Category: Genetics & Stem Cells Source Type: research

PDGF signaling from pharyngeal pouches promotes arch artery morphogenesis
Publication date: Available online 25 November 2019Source: Journal of Genetics and GenomicsAuthor(s): Aihua Mao, Mingming Zhang, Jie Liu, Yu Cao, Qiang WangAbstractThe great vessels of the heart originate from the pharyngeal arch arteries (PAAs). Anomalies of the PAAs often occur together with pharyngeal pouch malformations, but the reasons for this phenomenon are not fully understood. In the current study, we show that PDGF signaling derived from the pharyngeal pouches plays an important function in PAA vasculogenesis. During PAA development in zebrafish embryos, pdgfαa and pdgfαb are expressed in the developi...
Source: Journal of Genetics and Genomics - November 25, 2019 Category: Genetics & Stem Cells Source Type: research

Engineering guide RNA to reduce the off-target effects of CRISPR
Publication date: Available online 23 November 2019Source: Journal of Genetics and GenomicsAuthor(s): Jing Wu, Hao YinAbstractAs versatile and robust genome editing tools, clustered regularly interspaced short palindromic repeats (CRISPR) technologies have been broadly used in basic research, biotechnology and therapeutic development. Off-target mutagenesis by CRISPR systems has been demonstrated, and various methods have been developed to markedly increase their specificity. In this review, we highlight the efforts of producing and modifying guide RNA to minimize off-target activities, including sequence and structure des...
Source: Journal of Genetics and Genomics - November 23, 2019 Category: Genetics & Stem Cells Source Type: research

Advances in Detecting and Reducing Off-target Effects Generated by CRISPR-mediated Genome Editing
Publication date: Available online 22 November 2019Source: Journal of Genetics and GenomicsAuthor(s): Jin-Jing Li, Shunyan Hong, Wan-Jin Chen, Erwei Zuo, Hui YangAbstractCRISPR-mediated genome editing is a revolutionary technology for genome manipulation that utilizes the CRISPR-Cas systems and base editors. Currently, poor efficiency and off-target problems have impeded the application of CRISPR systems. The on-target efficiency has been improved in several advanced versions of CRISPR systems, whereas the off-target detection still remains a key challenge. Here, we outline the different versions of CRISPR systems and off-...
Source: Journal of Genetics and Genomics - November 22, 2019 Category: Genetics & Stem Cells Source Type: research

A behavioral paradigm to study the persistence of reward memory extinction in Drosophila
Publication date: Available online 16 November 2019Source: Journal of Genetics and GenomicsAuthor(s): Lingling Wang, Qi Yang, Binyan Lu, Lianzhang Wang, Yi Zhong, Qian Li (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - November 16, 2019 Category: Genetics & Stem Cells Source Type: research

Rapid breeding of pink-fruited tomato hybrids using the CRISPR/Cas9 system
Publication date: Available online 24 October 2019Source: Journal of Genetics and GenomicsAuthor(s): Tianxia Yang, Lei Deng, Wei Zhao, Ruoxi Zhang, Hongling Jiang, Zhibiao Ye, Chang-Bao Li, Chuanyou Li (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - November 14, 2019 Category: Genetics & Stem Cells Source Type: research

RASSF6-TRIM16 axis promotes cell proliferation, migration and invasion in esophageal squamous cell carcinoma
In this study, we performed immunohistochemistry (IHC) assay. The results show that RASSF6 is upregulated in ESCC and that the elevated expression level of RASSF6 is associated with lymph node metastasis and poor survival of ESCC patients. Consistent with the clinical observations, the upregulation of RASSF6 greatly promotes ESCC cell proliferation, migration and invasion as well as the cell cycle transition to G1/S phase in vitro. According to models in vivo, the downregulation of RASSF6 considerably inhibits ESCC tumor growth and lung metastasis. Mechanistically, RASSF6 negatively regulates the tumor suppressor tripartit...
Source: Journal of Genetics and Genomics - November 14, 2019 Category: Genetics & Stem Cells Source Type: research

Evolutionary selection on MDA5 and LGP2 in the chicken preserves antiviral competence in the absence of RIG-I
Publication date: Available online 5 November 2019Source: Journal of Genetics and GenomicsAuthor(s): Ling Xu, Dandan Yu, Yu Fan, Yi-Ping Liu, Yong-Gang Yao (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - November 6, 2019 Category: Genetics & Stem Cells Source Type: research

Embryonic lineage tracing with Procr-CreER marks balanced hematopoietic stem cell fate during entire mouse lifespan
In this study, we revealed that Procr expression was detected principally in phenotypical vascular endothelium co-expressing Dll4 and CD44 in the mid-gestation mouse embryos, and could enrich all the HSCs of the embryonic day 11.5 (E11.5) aorta-gonad-mesonephros (AGM) region. We then used a temporally restricted genetic tracing strategy to irreversibly label the Procr-expressing cells at E9.5. Interestingly, most labeled mature HSCs in multiple sites (such as AGM) around E11.5 were functionally categorized as lymphomyeloid-balanced HSCs assessed by direct transplantation. Furthermore, the labeled cells contributed to an av...
Source: Journal of Genetics and Genomics - November 6, 2019 Category: Genetics & Stem Cells Source Type: research

Histone modifications and their regulatory roles in plant development and environmental memory
Publication date: Available online 2 November 2019Source: Journal of Genetics and GenomicsAuthor(s): Ting Zhao, Zhenping Zhan, Danhua JiangAbstractPlants grow in dynamic environments where they receive diverse environmental signals. Swift and precise control of gene expression is essential for plants to align their development and metabolism with fluctuating surroundings. Modifications on histones serve as “histone code” to specify chromatin and gene activities. Different modifications execute distinct functions on the chromatin, promoting either active transcription or gene silencing. Histone writers, erasers,...
Source: Journal of Genetics and Genomics - November 4, 2019 Category: Genetics & Stem Cells Source Type: research

Off-target effects of cytidine base editor and adenine base editor: what can we do?
Publication date: Available online 2 November 2019Source: Journal of Genetics and GenomicsAuthor(s): Puping Liang, Jinkun Wen, Junjiu Huang (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - November 4, 2019 Category: Genetics & Stem Cells Source Type: research

Acquisition of functional neurons by direct conversion: Switching the developmental clock directly
Publication date: Available online 28 October 2019Source: Journal of Genetics and GenomicsAuthor(s): Shuangquan Chen, Juan Zhang, Dongming Zhang, Jianwei JiaoAbstractIdentifying approaches for treating neurodegeneration is a thorny task but is important for a growing number of patients. Researchers have focused on discovering the underlying molecular mechanisms of reprogramming and optimizing the technologies for acquiring neurons. Direct conversion is one of the most important processes for treating neurological disorders. Induced neurons (iNs) derived from direct conversion, which bypass the pluripotency stage, are more ...
Source: Journal of Genetics and Genomics - October 29, 2019 Category: Genetics & Stem Cells Source Type: research

Rapid breeding of pink-fruited tomato hybrids using CRISPR/Cas9 system
Publication date: Available online 24 October 2019Source: Journal of Genetics and GenomicsAuthor(s): Tianxia Yang, Lei Deng, Wei Zhao, Ruoxi Zhang, Hongling Jiang, Zhibiao Ye, Chang-Bao Li, Chuanyou Li (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - October 26, 2019 Category: Genetics & Stem Cells Source Type: research

Establishment and characterization of a complete set of Triticum durum-Thinopyrum elongatum monosomic addition lines with resistance to wheat fusarium head blight
Publication date: Available online 23 October 2019Source: Journal of Genetics and GenomicsAuthor(s): Jing Wang, Qinghua Shi, Xianrui Guo, Fangpu Han (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - October 23, 2019 Category: Genetics & Stem Cells Source Type: research

A highly efficient in vivo plasmid editing tool based on CRISPR-Cas12a and phage λ Red recombineering
Publication date: Available online 10 September 2019Source: Journal of Genetics and GenomicsAuthor(s): Yiman Geng, Hai-Qin Yan, Pei Li, Gai-Xian Ren, Xiao-Peng Guo, Peiqi Yin, Leiliang Zhang, Zhaohui Qian, Zhendong Zhao, Yi-Cheng Sun (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - October 11, 2019 Category: Genetics & Stem Cells Source Type: research

The p.(Pro170Leu) variant in NOG impairs noggin secretion and causes autosomal dominant congenital conductive hearing loss due to stapes ankylosis
Publication date: Available online 27 September 2019Source: Journal of Genetics and GenomicsAuthor(s): Yilai Shu, Lijun Wang, Xiaoting Cheng, Chayada Tangshewinsirikul, Weili Shi, Yasheng Yuan, Zhiqiang Yan, Huawei Li, Jun Shen, Bing Chen, Weiguo Zou (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - September 28, 2019 Category: Genetics & Stem Cells Source Type: research

Identification of transcriptional isoforms associated with survival in cancer patient
In this study we performed the first large-scale identification of transcriptional isoforms that are specifically associated with patient prognosis, even without gene-level association. These specific isoforms are defined as Transcripts Associated with Patient Prognosis (TAPP). Although a group of TAPPs are the principal isoforms of their genes with intact functional protein domains, another group of TAPPs lack important protein domains found in their canonical gene isoforms. This dichotomy in the distribution of protein domains may indicate different patterns of TAPP association with cancer. TAPPs in protein-coding genes,...
Source: Journal of Genetics and Genomics - September 25, 2019 Category: Genetics & Stem Cells Source Type: research

Temperature-sensitive cytoophidium assembly in Schizosaccharomyces pombe
Publication date: Available online 24 September 2019Source: Journal of Genetics and GenomicsAuthor(s): Jing Zhang, Ji-Long LiuAbstractThe metabolic enzyme CTP synthase (CTPS) is able to compartmentalize into filaments, termed cytoophidia, in a variety of organisms including bacteria, budding yeast, fission yeast, fruit flies and mammals. A previous study in budding yeast shows that the filament-forming process of CTPS is not sensitive to temperature shift. Here we study CTPS filamentation in the fission yeast Schizosaccharomyces pombe. To our surprise, we find that both the length and the occurrence of cytoophidia in S. po...
Source: Journal of Genetics and Genomics - September 24, 2019 Category: Genetics & Stem Cells Source Type: research

Examining the practical limits of batch effect-correction algorithms: When should you care about batch effects?
Publication date: Available online 20 September 2019Source: Journal of Genetics and GenomicsAuthor(s): Longjian Zhou, Andrew Chi-Hau Sue, Wilson Wen Bin GohAbstractBatch effects are technical sources of variation and can confound analysis. While many performance ranking exercises have been conducted to establish the best batch effect-correction algorithm (BECA), we hold the viewpoint that the notion of best is context-dependent. Moreover, alternative questions beyond the simplistic notion of “best” are also interesting: are BECAs robust against various degrees of confounding and if so, what is the limit? Using ...
Source: Journal of Genetics and Genomics - September 21, 2019 Category: Genetics & Stem Cells Source Type: research

SERCA regulates collective cell migration by maintaining cytoplasmic Ca2+ homeostasis
Publication date: Available online 20 September 2019Source: Journal of Genetics and GenomicsAuthor(s): Xuan Guo, Jun Luo, Heng Wang, Jiong Chen (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - September 21, 2019 Category: Genetics & Stem Cells Source Type: research

A highly efficient in vivo plasmid editing tool based on CRISPR-Cas12a and phage λ Red recombineering
Publication date: Available online 10 September 2019Source: Journal of Genetics and GenomicsAuthor(s): Yiman Geng, Hai-Qin Yan, Pei Li, Gai-Xian Ren, Xiao-Peng Guo, Peiqi Yin, Leiliang Zhang, Zhaohui Qian, Zhendong Zhao, Yi-Cheng Sun (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - September 10, 2019 Category: Genetics & Stem Cells Source Type: research

Blocking CD38-driven fratricide among T cells enables effective antitumor activity by CD38-specific chimeric antigen receptor T cells
In conclusion, these findings will help to further enhance the expansion, persistence and function of anti-CD38 CAR T cells in subsequent clinical trials. (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - August 14, 2019 Category: Genetics & Stem Cells Source Type: research

Establishment of wheat-Thinopyrum ponticum translocation lines with resistance to Puccinia graminis f. sp. tritici Ug99
Publication date: Available online 13 August 2019Source: Journal of Genetics and GenomicsAuthor(s): Hongwei Li, Willem H.P. Boshoff, Zacharias A. Pretorius, Qi Zheng, Bin Li, Zhensheng Li (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - August 14, 2019 Category: Genetics & Stem Cells Source Type: research

The expression tractability of biological traits shaped by natural selection
Publication date: Available online 13 August 2019Source: Journal of Genetics and GenomicsAuthor(s): Li Liu, Jianguo Wang, Jianrong Yang, Feng Wang, Xionglei HeAbstractUnderstanding how gene expression is translated to phenotype is central to modern molecular biology, and the success is contingent on the intrinsic tractability of the specific traits under examination. However, an a priori estimate of trait tractability from the perspective of gene expression is unavailable. Motivated by the concept of entropy in a thermodynamic system, we here propose such an estimate (ST) by gauging the number (N) of expression states that...
Source: Journal of Genetics and Genomics - August 14, 2019 Category: Genetics & Stem Cells Source Type: research

OSBPL2-disrupted pigs recapitulate dual features of human hearing loss and hypercholesterolaemia
Publication date: Available online 13 August 2019Source: Journal of Genetics and GenomicsAuthor(s): Jun Yao, Huasha Zeng, Min Zhang, Qinjun Wei, Ying Wang, Haiyuan Yang, Yajie Lu, Rongfeng Li, Qiang Xiong, Lining Zhang, Zhibin Chen, Guangqian Xing, Xin Cao, Yifan DaiAbstractOxysterol binding protein like 2 (OSBPL2), an important regulator in cellular lipid metabolism and transport, was identified as a novel deafness-causative gene in our previous work. To resemble the phenotypic features of OSBPL2 mutation in animal models and elucidate the potential genotype-phenotype associations, the OSBPL2-disrupted Bama miniature (BM)...
Source: Journal of Genetics and Genomics - August 14, 2019 Category: Genetics & Stem Cells Source Type: research

Progressive heterosis in genetically defined tetraploid maize
In this study, four artificially induced maize tetraploids, directly derived from standard inbred lines, were crossed in pairs to create two single-cross hybrids. These hybrids were then crossed to create double-cross hybrids containing genetic material from all four original lines. Replicated field-based phenotyping of the materials over four years indicated a strong progressive heterosis phenotype in tetraploids but not in their diploid counterparts. In particular, the above ground dry weight phenotype of double-cross tetraploid hybrids was on average 34 % and 56% heavier than that of the single-cross tetraploid hybrids ...
Source: Journal of Genetics and Genomics - August 10, 2019 Category: Genetics & Stem Cells Source Type: research

Gut microbiota from end-stage renal disease patients disrupt gut barrier function by excessive production of phenol
Publication date: Available online 2 August 2019Source: Journal of Genetics and GenomicsAuthor(s): Xifan Wang, Yanling Hao, Xiaoxue Liu, Shoujuan Yu, Weibo Zhang, Songtao Yang, Zhengquan Yu, Fazheng Ren (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - August 3, 2019 Category: Genetics & Stem Cells Source Type: research

Mapping quantitative trait loci using binned genotypes
Publication date: Available online 23 July 2019Source: Journal of Genetics and GenomicsAuthor(s): Wen Yao, Guangwei Li, Yanru Cui, Yiming Yu, Qifa Zhang, Shizhong XuAbstractPrecise mapping of quantitative trait loci (QTLs) is critical for assessing the genetic effects and identification of candidate genes for quantitative traits. Interval and composite interval mappings have been the methods of choice for several decades, which have provided tools for identifying genomic regions harboring causal genes for quantitative traits. Historically, the concept was developed on the basis of sparse marker maps where genotypes of loci...
Source: Journal of Genetics and Genomics - July 25, 2019 Category: Genetics & Stem Cells Source Type: research

Production of non-mosaic genome edited porcine embryos by injection of CRISPR/Cas9 into germinal vesicle oocytes
In conclusion, GVGE might be a valuable method for the generation of non-mosaic maternal allele edited F0 embryos in a short simple step. (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - July 22, 2019 Category: Genetics & Stem Cells Source Type: research

Gene redundancy and gene compensation: an updated view
Publication date: Available online 19 July 2019Source: Journal of Genetics and GenomicsAuthor(s): Jinrong PengAbstractGene knockdown approaches using antisense oligo nucleotides or analogs such as siRNAs and morpholinos have been widely adopted to study gene functions although the off-target issue has been always a concern in these studies. On the other hand, classic genetic analysis relies on the availability of loss-of-function or gain-of-function mutants. The fast development of gene editing technologies such as TALEN and CRISPR/Cas9 has greatly facilitated the generation of null mutants for the functional studies of ta...
Source: Journal of Genetics and Genomics - July 21, 2019 Category: Genetics & Stem Cells Source Type: research

Rare variants in novel and known genes associated with primary angle closure glaucoma based on whole exome sequencing of 549 probands
Publication date: Available online 10 July 2019Source: Journal of Genetics and GenomicsAuthor(s): Wenmin Sun, Xueshan Xiao, Shiqiang Li, Jiamin Ouyang, Xueqing Li, Xiaoyun Jia, Xing Liu, Qingjiong Zhang (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - July 10, 2019 Category: Genetics & Stem Cells Source Type: research

Pyramiding of the dep1-1 and NAL1NJ6 alleles achieves sustainable improvements in nitrogen-use efficiency and grain yield in japonica rice breeding
Publication date: Available online 1 June 2019Source: Journal of Genetics and GenomicsAuthor(s): Xiaopeng Xu, Kun Wu, Ruineng Xu, Jianping Yu, Jing Wang, Ying Zhao, Yun Wang, Wenzhen Song, Shuoxun Wang, Zhi Gao, Yongjia Zhong, Xinxin Li, Hong Liao, Xiangdong Fu (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - June 30, 2019 Category: Genetics & Stem Cells Source Type: research

Dissecting PCNA function with a systematically designed mutant library in yeast
Publication date: Available online 24 June 2019Source: Journal of Genetics and GenomicsAuthor(s): Qingwen Jiang, Weimin Zhang, Chenghao Liu, Yicong Lin, Qingyu Wu, Junbiao DaiAbstractProliferating cell nuclear antigen (PCNA), encoded by POL30 in Saccharomyces cerevisiae, is a key component of DNA metabolism. Here, a library consisting of 304 PCNA mutants was designed and constructed to probe the contribution of each residue to the biological function of PCNA. Five regions with elevated sensitivity to DNA damaging reagents were identified using high-throughput phenotype screening. Using a series of genetic and biochemical a...
Source: Journal of Genetics and Genomics - June 25, 2019 Category: Genetics & Stem Cells Source Type: research

OsGCD1, a novel player in rice intine construction
Publication date: Available online 25 June 2019Source: Journal of Genetics and GenomicsAuthor(s): Xiaorong Huang, Mengmeng Run, Meng-Xiang Sun (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - June 25, 2019 Category: Genetics & Stem Cells Source Type: research

Nlrc3-like is required for microglia maintenance in zebrafish
Publication date: Available online 22 June 2019Source: Journal of Genetics and GenomicsAuthor(s): Tienan Wang, Bo Yan, Liang Lou, Xi Lin, Tao Yu, Shuting Wu, Qing Lu, Wei Liu, Zhibin Huang, Mingjie Zhang, Wenqing Zhang, Zilong WenAbstractMicroglia are tissue-resident macrophages resided in the central nervous system (CNS) and play critical roles in removing cellular debris and infectious agents as well as regulating neurogenesis and neuronal activities. Yet, the molecular basis underlying the establishment of microglia pool and the maintenance of their homeostasis in the CNS remain largely undefined. Here we report the ide...
Source: Journal of Genetics and Genomics - June 24, 2019 Category: Genetics & Stem Cells Source Type: research

A modified high-efficiency thermal asymmetric interlaced PCR method for amplifying long unknown flanking sequences
Publication date: Available online 23 June 2019Source: Journal of Genetics and GenomicsAuthor(s): Jiantao Tan, Qi Gong, Suize Yu, Yuke Hou, Dongchang Zeng, Qinlong Zhu, Yao-Guang Liu (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - June 24, 2019 Category: Genetics & Stem Cells Source Type: research

Mutations in TP53, ZNF750, and RB1 typify ocular sebaceous carcinoma
Publication date: Available online 21 June 2019Source: Journal of Genetics and GenomicsAuthor(s): Yongyang Bao, J. Eva Selfridge, Janet Wang, Yiqing Zhao, Junqi Cui, Kishore Guda, Zhenghe Wang, Yanbo Zhu (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - June 21, 2019 Category: Genetics & Stem Cells Source Type: research

RSBP15 interacts with and stabilizes dRSPH3 during sperm axoneme assembly in Drosophila
Publication date: Available online 1 June 2019Source: Journal of Genetics and GenomicsAuthor(s): Ya Wang, Rui Xu, Yiwei Cheng, Haowei Cao, Zibin Wang, Tianyu Zhu, Jiayin Jiang, Hao Zhang, Chang Wang, Lin Qi, Mingxi Liu, Xuejiang Guo, Juan Huang, Jiahao ShaAbstractFlagellum in sperm is composed of over 200 different proteins and is essential for sperm motility. In particular, defects in the assembly of the radial spoke in the flagellum result in male infertility due to loss of sperm motility. However, mechanisms regulating radial spoke assembly remain unclear in metazoans. Here, we identified a novel Drosophila protein radi...
Source: Journal of Genetics and Genomics - June 3, 2019 Category: Genetics & Stem Cells Source Type: research

Pyramiding of the dep1-1 and NAL1NJ6 alleles achieves sustainable improvements in nitrogen use efficiency and grain yield in japonica rice breeding
Publication date: Available online 1 June 2019Source: Journal of Genetics and GenomicsAuthor(s): Xiaopeng Xu, Kun Wu, Ruineng Xu, Jianping Yu, Jing Wang, Ying Zhao, Yun Wang, Wenzhen Song, Shuoxun Wang, Zhi Gao, Yongjia Zhong, Xinxin Li, Hong Liao, Xiangdong Fu (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - June 3, 2019 Category: Genetics & Stem Cells Source Type: research

Genetic analysis and preimplantation genetic diagnosis of Chinese Marfan syndrome patients
Publication date: Available online 24 May 2019Source: Journal of Genetics and GenomicsAuthor(s): Meng Qin, Xiaohui Zhu, Zhe Zhang, Xuemin Li, Zhiqiang Yan, Yuqian Wang, Shuo Guan, Yihua He, Wenxin Zhang, Liying Yan, Jie Qiao, Xu Zhi (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - May 25, 2019 Category: Genetics & Stem Cells Source Type: research

Rare inherited missense variants of POGZ associate with autism risk and disrupt neuronal development
Publication date: Available online 18 May 2019Source: Journal of Genetics and GenomicsAuthor(s): Wenjing Zhao, Jieqiong Tan, Tengfei Zhu, Jianjun Ou, Ying Li, Lu Shen, Huidan Wu, Lin Han, Yanling Liu, Xiangbin Jia, Ting Bai, Honghui Li, Xiaoyan Ke, Jingping Zhao, Xiaobing Zou, Zhengmao Hu, Hui Guo, Kun XiaAbstractExcess de novo likely gene-disruptive and missense variants within dozens of genes have been identified in autism spectrum disorder (ASD) and other neurodevelopmental disorders. However, many rare inherited missense variants of these high-risk genes have not been thoroughly evaluated. Here, we analyzed the rare mi...
Source: Journal of Genetics and Genomics - May 18, 2019 Category: Genetics & Stem Cells Source Type: research

mTOR/miR-145-regulated exosomal GOLM1 promotes hepatocellular carcinoma through augmented GSK-3β/MMPs
In this study, we demonstrated that the mammalian target of rapamycin (mTOR) was a negative regulator of microRNA-145 (miR-145) expression. miR-145 inhibited GOLM1 expression by targeting a coding sequence of GOLM1 gene. GOLM1 and miR-145 were inversely correlated in human HCC tissues. GOLM1-enriched exosomes activated the glycogen synthase kinase-3β/matrix metalloproteinases (GSK-3β/MMPs) signaling axis of recipient cells and accelerated cell proliferation and migration. In contrast, miR-145 suppressed tumorigenesis and metastasis. We suggest that mTOR/miR-145/GOLM1 signaling pathway should be targeted for HCC t...
Source: Journal of Genetics and Genomics - May 18, 2019 Category: Genetics & Stem Cells Source Type: research

Ciliopathy-associated proteins are involved in vesicle distribution in sensory cilia
Publication date: Available online 15 May 2019Source: Journal of Genetics and GenomicsAuthor(s): Ming Li, Wanzhong He, Wei Li, Guangshuo Ou (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - May 16, 2019 Category: Genetics & Stem Cells Source Type: research

Understanding human diseases using Drosophila
Publication date: Available online 26 April 2019Source: Journal of Genetics and GenomicsAuthor(s): Jun-Yuan Ji, Chun Han, Wu-Min Deng (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - April 27, 2019 Category: Genetics & Stem Cells Source Type: research

Organelle aging: lessons from model organisms
Publication date: Available online 25 April 2019Source: Journal of Genetics and GenomicsAuthor(s): Mark Bouska, Kerui Huang, Ping Kang, Hua BaiAbstractMost cellular processes descend into failure during aging. While a large collection of longevity pathways has been identified in the past decades, the mechanism for age-related decline of cellular homeostasis and organelle function remains largely unsolved. It is known that many organelles undergo structural and functional changes during normal aging, which significantly contributes to the decline of tissue function at old ages. Since recent studies have revealed an emerging...
Source: Journal of Genetics and Genomics - April 25, 2019 Category: Genetics & Stem Cells Source Type: research

Understanding the importance of autophagy in human diseases using Drosophila
Publication date: Available online 23 April 2019Source: Journal of Genetics and GenomicsAuthor(s): Arindam Bhattacharjee, Áron Szabó, Tamás Csizmadia, Hajnalka Laczkó-Dobos, Gábor JuhászAbstractAutophagy is a lysosome-dependent intracellular degradation pathway that has been implicated in the pathogenesis of various human diseases, either positively or negatively impacting disease outcomes depending on the specific context. The majority of medical conditions including cancer, neurodegenerative diseases, infections and immune system disorders and inflammatory bowel disease could pro...
Source: Journal of Genetics and Genomics - April 25, 2019 Category: Genetics & Stem Cells Source Type: research

Human mitochondrial DNA diseases and Drosophila models
Publication date: Available online 23 April 2019Source: Journal of Genetics and GenomicsAuthor(s): Zhe Chen, Fan Zhang, Hong XuAbstractMutations that disrupt the mitochondrial genome cause a number of human diseases whose phenotypic presentation varies widely among tissues and individuals. This variability owes in part to the unconventional genetics of mitochondrial DNA (mtDNA), which includes polyploidy, maternal inheritance and dependence on nuclear-encoded factors. The recent development of genetic tools for manipulating mitochondrial genome in Drosophila melanogaster renders this powerful model organism an attractive a...
Source: Journal of Genetics and Genomics - April 23, 2019 Category: Genetics & Stem Cells Source Type: research

Die in pieces: How Drosophila sheds light on neurite degeneration and clearance
Publication date: Available online 23 April 2019Source: Journal of Genetics and GenomicsAuthor(s): Maria L. Sapar, Chun HanAbstractDendrites and axons are delicate neuronal membrane extensions that undergo degeneration after physical injuries. In neurodegenerative diseases, these processes often degenerate prior to neuronal death. Understanding the mechanisms of neurite degeneration has been an intense focus of neurobiology research in the last two decades. As a result, many discoveries have been made in the molecular pathways that lead to neurite degeneration and the cell-cell interactions responsible for the subsequent c...
Source: Journal of Genetics and Genomics - April 23, 2019 Category: Genetics & Stem Cells Source Type: research