Crystal structures of the cell-division protein FtsZ from Klebsiella pneumoniae and Escherichia coli

In this study, crystal structures of the enzymatic domains of FtsZ from Klebsiella pneumoniae (KpFtsZ) and Escherichia coli (EcFtsZ) were determined at 1.75 and 2.50   Å resolution, respectively. Both FtsZs form straight protofilaments in the crystals, and the two structures adopted relaxed (R) conformations. The T3 loop, which is   involved in GTP/GDP binding and FtsZ assembly/disassembly, adopted a unique open conformation in KpFtsZ, while the T3 loop of EcFtsZ was partially disordered. The crystal structure of EcFtsZ can explain the results from previous functional analyses using EcFtsZ mutants.
Source: Acta Crystallographica Section F - Category: Biochemistry Authors: Tags: cell division FtsZ Escherichia coli Klebsiella pneumoniae research communications Source Type: research