In-Vitro Antioxidant, Antimutagenic and Cancer Cell Growth Inhibition Activities of Rhododendron arboreum Leaves and Flowers

Publication date: Available online 3 February 2020Source: Saudi Journal of Biological SciencesAuthor(s): Vandana Gautam, Anket Sharma, Saroj Arora, Renu Bhardwaj, Ajaz Ahmad, Bilal Ahamad, Parvaiz AhmadAbstractIn the current investigation, the active principles of the methanol extracts of Rhododendron arboreum leaves (MEL) and flowers (MEF) were investigated with the help of ultra-high performance liquid chromatography (UHPLC), amino acid analyzer and gas chromatography mass spectrometry (GC-MS). UHPLC revealed different polyphenols present in the extracts. GC-MS identified 20 phytochemicals in leaves and 17 in the flowers, whereas, amino acid analyzer confirmed 11 amino acids in leaves and 10 in the flowers. The extracts were subjected to the investigation of biological activity through analysis of antioxidant activity in different in vitro assays, antimutagenic activity in Ames assay and cancer cell growth inhibition activity by MTT (3-4,5 dimethylthiazol-2,5 diphenyltetrazolium bromide) assay. MEL showed higher antioxidant activity in lipid peroxidation inhibition assay (95.32±0.37%) than MEF (77.09±4.17%) with IC50103.6µg/ml for MEL and 271.17µg/ml for MEF. In nitric oxide scavenging assay, an activity of 94.46±0.32% (IC50 150.13) was observed in MEF followed by 83.71±0.74% (IC50 179.52) in MEL. The antimutagenic activity of both the extracts was evaluated against sodium azide, 4-nitro-O-phenylenediamine and 2-aminofluorene mutagens in TA-98 and TA-100strains of Sal...
Source: Saudi Journal of Biological Sciences - Category: Biology Source Type: research