Flotillin and AP2A1/2 promote insulin-like growth factor receptor-1 association with clathrin and internalization in primary human keratinocytes

Insulin-like growth factor-1 (IGF-1) receptor (IGF1R) signaling promotes keratinocyte proliferation, migration, and survival. However, the mechanism of IGF1R endocytosis in normal keratinocytes remains unclear. Confocal, super resolution structured illumination microscopy (SIM), total internal reflection fluorescence/TIRF microscopy, and coimmunoprecipitation studies reveal that IGF1R associates with flotillin-1, which currently has no known role in normal receptor tyrosine kinase endocytosis, under basal conditions in monolayer keratinocyte cultures.

Source: Journal of Investigative Dermatology - February 2, 2020 Category: Dermatology Authors: Duncan Hieu M. Dam, Sophia A. Jelsma, Jeong Min Yu, Haoming Liu, Betty Kong, Amy S. Paller Tags: Original Article Source Type: research