Effect of surface chemistry on hMSC growth under xeno-free conditions

Publication date: Available online 30 January 2020Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Maura Cimino, Paula Parreira, Sílvia Bidarra, Raquel M. Gonçalves, Cristina C. Barrias, M. Cristina L. MartinsAbstractHuman mesenchymal stem/stromal cells (hMSC) are promising therapeutic agents for regenerative medicine. However, therapeutic doses necessary for clinical application require in vitro expansion, ideally under Xeno-Free conditions (XF) to avoid the use of foetal bovine serum (FBS). We previously reported that hMSCs could be expanded using a pharmaceutical-grade human plasma-derived supplement for cell culture (SCC, Plastem®) combined with bFGF and TGFβ1, on fibronectin (Fn)-coated surfaces.hMSCs expansion may also be affected by the chemistry of the culture surface, which modulates protein adsorption at the cell-material interface and, consequently, cell behavior.This work aimed to evaluate the effect of surface chemistry on hMSCs behaviour in SCC-based XF media. For that, self-assembled monolayers (SAMs) with hydrophobic (-CH3) and hydrophilic (neutral -OH, positively charged -NH3+ and negatively charged -COO-) groups were used as model surfaces.Under XF conditions, Fn coating showed to be necessary to improve hMSC adhesion (4 h) onto all surfaces, except for OH-SAMs, probably due to a low protein adsorption capacity characteristic of this surface. In terms of cell metabolic activity (5 days) on Fn-coated surfaces, an increase over time under XF cond...

Source: Colloids and Surfaces B: Biointerfaces - February 2, 2020 Category: Biochemistry Source Type: research