Development of novel highly sensitive methods to detect endogenous cGAMP in cells and tissue.

Development of novel highly sensitive methods to detect endogenous cGAMP in cells and tissue. J Immunol Methods. 2020 Jan 23;:112751 Authors: Miyakawa S, Okui T, Shiraishi T, Yoshihara T, Hirayama M, Satomi Y, Hamada T, Nishida M, Akimoto C, Sato S Abstract Intracellular DNA triggers interferon release during the innate immune response. Cyclic GMP-AMP synthase (cGAS) senses intracellular double-stranded DNA not only in response to viral infection but also under autoimmune conditions. Measuring the levels of cyclic GMP-AMP (cGAMP) as a second messenger of cGAS activation is important to elucidate the physiological and pathological roles of cGAS. Therefore, we generated monoclonal antibodies against cGAMP using hybridoma technology to test antibody specificity and establish methods to detect intracellular cGAMP. The resulting cGAMP-specific antibody enabled the development of a time-resolved fluorescence energy transfer assay with a quantifiable range of 0.1 nM to 100 nM cGAMP. Using this assay, we detected cellular and tissue cGAMP. We confirmed that the cGAMP antibody successfully targeted intracellular cGAMP through immunocytochemical analyses. These results demonstrated that the cGAMP antibody is a powerful tool that allows determining cGAS involvement in autoimmunity and disease pathology at the cell and tissue levels. PMID: 31982420 [PubMed - as supplied by publisher]
Source: Journal of Immunological Methods - Category: Allergy & Immunology Authors: Tags: J Immunol Methods Source Type: research