In silico and in vitro comparison of nicotinamide adenine dinucleotide phosphate dependent xylose reductase rossmaan fold in Debaryomycetaceae yeast family

This study explore the in silico and in vitro comparative analysis of cofactor NADP binding mechanism in rossmann fold of isolated M. caribbica and other xylose fermenting Debaryomycetaceae family yeast. The rossmaan fold analysis of M. caribbica and Debaryomycetaceae family of yeast revealed, xylose fermenting organisms Scheffersomyces stipitis, Debaryomyces hansenii, Debaryomyces nepalensis, Candida parapsilosis, Candida maltose has divergence structure of rosmann fold. Whereas M. caribbica and Calamus tenuis has unique structure of rossmann fold. Also, the identified rossmann fold of Debaryomycetaceae family yeast and isolated M. caribbica are conserved to interact with cofactor NADP by H2 bonding, which displays the close proximity evolutionary relationship of XR enzyme in Debaryomycetaceae family yeast. Annotation analysis showed the isolated M. caribbica has polyols fermentation for xylitol production.Graphical abstract

Source: Biocatalysis and Agricultural Biotechnology - January 23, 2020 Category: Biotechnology Source Type: research