In silico and in vitro comparison of nicotinamide adenine dinucleotide phosphate dependent xylose reductase rossmaan fold in Debaryomycetaceae yeast family

This study explore the in silico and in vitro comparative analysis of cofactor NADP binding mechanism in rossmann fold of isolated M. caribbica and other xylose fermenting Debaryomycetaceae family yeast. The rossmaan fold analysis of M. caribbica and Debaryomycetaceae family of yeast revealed, xylose fermenting organisms Scheffersomyces stipitis, Debaryomyces hansenii, Debaryomyces nepalensis, Candida parapsilosis, Candida maltose has divergence structure of rosmann fold. Whereas M. caribbica and Calamus tenuis has unique structure of rossmann fold. Also, the identified rossmann fold of Debaryomycetaceae family yeast and isolated M. caribbica are conserved to interact with cofactor NADP by H2 bonding, which displays the close proximity evolutionary relationship of XR enzyme in Debaryomycetaceae family yeast. Annotation analysis showed the isolated M. caribbica has polyols fermentation for xylitol production.Graphical abstract
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research

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Publication date: Available online 11 February 2020Source: Journal de Mycologie MédicaleAuthor(s): C.S. Shinobu-Mesquita, E. Martins, J.B. Júnior, P. de Souza Bonfim-Mendonça, M.S.S. Felipe, É.S. Kioshima, T.I.E. Svidzinski
Source: Journal of Medical Mycology - Category: Biology Source Type: research
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Source: Evidence-based Complementary and Alternative Medicine - Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research
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Source: Microbial Drug Resistance - Category: Microbiology Authors: Source Type: research
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Source: JAMA - Category: General Medicine Source Type: research
Candida oleophila is an effective biocontrol agent used to control post-harvest diseases of fruits and vegetables. C. oleophila I-182 was the active agent used in the first-generation yeast-based commercial product, Aspire®, for post-harvest disease management. Several action modes, like competition for nutrients and space, induction of pathogenesis-related genes in host tissues, and production of extracellular lytic enzymes, have been demonstrated for the biological control activity exhibited by C. oleophila through which it inhibits post-harvest pathogens. In the present study, the whole genome of C. oleophila I-182 ...
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