Artemargyinolide E, a new sesquiterpene lactone from Artemisia argyi inhibits inflammatory responses via down-regulating NF-κB signaling pathway

Publication date: April 2020Source: Phytochemistry Letters, Volume 36Author(s): Lai-Bin Zhang, Hui-Hui Zhu, Li-Min Guo, Jie-Li LvAbstractArtemargyinolide E (AE), a new sesquiterpene lactone was isolated from the traditional Chinese medicine Artemisia argyi. Its structure was elucidated based on the extensive analysis of spectroscopic data. The absolute configuration of AE was assigned by X-ray crystallographic analysis. The effect of AE and its mechanism on lipopolysaccharide (LPS)-induced inflammatory responses in macrophages were investigated. AE displayed the inhibitory activity on the production of nitrogen oxide (NO) in LPS-induced RAW 264.7 macrophages with an IC50 value of 17.20 ± 0.55 μM. Furthermore, AE intensively suppressed the secretion of key pro-inflammatory factors and chemokines, including tumor necrosis factor-α (TNF-α), prostaglandin E2 (PGE2) and interleukin-1β (IL-1β). It also significantly down-regulated the protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Further results revealed that AE inhibited LPS-induced phosphorylation of inhibitor of κBα (IκBα) and nuclear factor (NF)-κB p65, and suppressed the translocation of NF-κB p65 from the cytoplasm to the nucleus. These results suggest that AE could inhibit inflammatory responses in LPS-induced RAW 264.7 macrophages via down-regulating NF-κB signaling pathway.Graphical abstract
Source: Phytochemistry Letters - Category: Chemistry Source Type: research