GSE143764 Involvement of transcription elongation factor GreA in Mycobacterium tuberculosis viability, antibiotic susceptibility, and intracellular fitness
Contributors : Siyuan Feng ; Guobao TianSeries Type : Expression profiling by high throughput sequencing ; Genome binding/occupancy profiling by high throughput sequencingOrganism : Mycolicibacterium smegmatis MC2 155To understand how GreA may affect gene expression at the transcriptional level, we performed whole-transcriptome RNA-seq analyses.To examine whether GreA binds to specific chromosomal regions, we performed chromatin immunoprecipitation followed by deep sequencing (ChIP-seq) and then sought to verify the binding mode of GreA. ChIP-seq experiments were performed by using M. smegmatis strains producing GreA proteins fused with two repeats of the FLAG epitope (FLAG2). GreA-FLAG2-DNA nucleoprotein complexes fixed with formaldehyde in the log phase of growth were immunoprecipitated using magnetic beads. To exclude unspecific interactions with magnetic beads, we used a WT strain lacking the FLAG epitope as a negative control for our ChIP-seq experiments. Enriched regions were determined by comparison to the background noise level, which was estimated versus the input DNA of each ChIP-Seq replicate.Disrupting greA resulted in the differential regulation of 195 genes in M. smegmatis during the exponential growth. Among these, 28 were positively regulated and 167 were negatively regulated.The identified GreA-FLAG2 binding sites included 1005 ChIP-seq peaks that were confirmed in two biological replicates and absent in WT strain.
Sunil Kumar RainaJournal of Global Infectious Diseases 2020 12(1):3-4
Conclusion: High initial loss to follow-up, delay in treatment initiation, and poor treatment outcome among ACF patients are a major concern. The study results call for active follow-up after diagnosis and close monitoring during treatment for patients detected by ACF.
Very old (aged ≥80 years) adults constitute an increasing proportion of the global population. Currently, this subgroup of patients represents an important percentage of patients admitted to the intensive care unit. Community-acquired pneumonia (CAP) frequently affects very old adults. However, there are no specific recommendations for the management of critically ill very old CAP patients. Multiple morbidities, polypharmacy, immunosenescence and frailty contribute to an increased risk of pneumonia in this population. CAP in critically ill very old patients is associated with higher short- and long-term mortality; ...
Pneumonia has been a serious cause of morbidity and mortality for many years and, more than 100 years ago, Sir William Osler referred to it as "the natural enemy of the old man" . Many studies have documented that elderly individuals have a higher frequency of illness, increased mortality and more subtle clinical features compared to younger populations. This too was known by Osler who commented that "it is not improbable that debility lowers the vitality and renders the individual susceptible" and when the illness occurs, it can be "without chill; the cough and expectoration are slight&quo...
Publication date: Available online 17 February 2020Source: Clinical Epidemiology and Global HealthAuthor(s): Krishna Prasad Acharya, Niran Adhikari, Muhammad TariqAbstractThe agro-climatic conditions, socio-economic situation, poor sanitation, low hygiene and health standards in Nepal are major contributing factors to the emergence of scrub typhus in Nepal. These outbreaks, along with poor diagnostic facilities, can lead to severe economic losses in a resource-limited country like Nepal. Diseases like avian influenza, leptospirosis, brucellosis, tuberculosis and rabies, that cause heavy socio-economic burden, have received...
To undertake a systematic review and meta-analysis of the diagnostic performance of CT for differentiating peritoneal tuberculosis (PTB) from peritoneal carcinomatosis (PC).
A 45-year-old African American man with HIV presented to an outside hospital two months prior to the current hospitalization with unsteady gait and left hemiparesis. Magnetic resonance imaging (MRI) of the brain demonstrated a left postcentral gyrus and two left temporal lobe enhancing lesions. Mycobacterium tuberculosis polymerase chain reaction was negative, however, he had a history of exposure to a family member with tuberculosis and was consequently started on rifampin, isoniazid, pyrazinamide, and ethambutol (RIPE) as well as antiretroviral therapy (ART).
Conditions: Tuberculosis; Tuberculosis Infection Interventions: Other: Multiple blood samples; Other: Single blood sample Sponsor: Hospices Civils de Lyon Recruiting