Loop-mediated amplification as promising on-site detection approach for Legionella pneumophila and Legionella spp.

Loop-mediated amplification as promising on-site detection approach for Legionella pneumophila and Legionella spp. Appl Microbiol Biotechnol. 2019 Dec 12;: Authors: Reuter C, Slesiona N, Hentschel S, Aehlig O, Breitenstein A, Csáki A, Henkel T, Fritzsche W Abstract Recently Legionella pneumophila is the main causative waterborne organism of severe respiratory infections. Additionally, other Legionella species are documented as human pathogens. In our work, we describe a rapid detection method which combines two advantages for sensitive and specific detection of the genus Legionella: the fast isothermal amplification method "Loop-mediated isothermal AMPlification" (LAMP), and a colorimetric detection method using the metal indicator hydroxynaphtol blue (HBN) which allows to determine an optical signal with a simple readout (with the naked eye). Moreover, we present two approaches for minimizing the assay volume using a stationary microchip LAMP and droplet digital-based LAMP (ddLAMP) as promising highly sensitive setups. PMID: 31832709 [PubMed - as supplied by publisher]
Source: Applied Microbiology and Biotechnology - Category: Microbiology Authors: Tags: Appl Microbiol Biotechnol Source Type: research