Differential adsorption of analyte and deuterated analogue onto the rotor seal of the injector in high-performance liquid chromatography/electrospray ionisation mass spectrometry.

CONCLUSIONS: This study has demonstrated the problem of differential adsorption onto HPLC Vespel rotor seals, and that the use of a stable isotope analogue as internal standard does not guarantee the constancy of the analyte/internal response ratio in quantitative methods. The partial solution was to work at much higher concentration where adsorption, whilst still apparent, was relatively insignificant.
PMID: 31800127 [PubMed - as supplied by publisher]

Source: Rapid Communications in Mass Spectrometry : RCM - December 3, 2019 Category: Chemistry Authors: Lord GA Tags: Rapid Commun Mass Spectrom Source Type: research