Next-generation interactomics: considerations for the use of co-elution to measure protein interaction networks.

Next-generation interactomics: considerations for the use of co-elution to measure protein interaction networks. Mol Cell Proteomics. 2019 Dec 02;: Authors: Salas D, Stacey RG, Akinlaja M, Foster LJ Abstract Understanding how proteins interact is crucial to understanding cellular processes. Among the available interactome mapping methods, co-elution stands out as a method that is simultaneous in nature and capable of identifying interactions between all the proteins detected in a sample. The general workflow in co-elution methods involves the mild extraction of protein complexes and their separation into a number of fractions, across which proteins bound together in the same complex will show similar co-elution profiles when analyzed appropriately. In this review we discuss the different separation, quantification and bioinformatic strategies used in co-elution studies, and the important considerations in designing these studies. The benefits of co-elution versus other methods makes it a valuable starting point when asking questions that involve the perturbation of the interactome. PMID: 31792070 [PubMed - as supplied by publisher]
Source: Molecular and Cellular Proteomics : MCP - Category: Molecular Biology Authors: Tags: Mol Cell Proteomics Source Type: research