Biocontrol potential of the endophytic Bacillus amyloliquefaciens YN201732 against tobacco powdery mildew and its growth promotion

Publication date: Available online 28 November 2019Source: Biological ControlAuthor(s): Rong Jiao, Shahzad Munir, Pengfei He, Huanwen Yang, Yixin Wu, Junwei Wang, Yongzhan Cai, Ge Wang, Yueqiu HeAbstractThe effectiveness of Bacillus amyloliquefaciens YN201732, a beneficial endophyte isolated from tobacco seeds was explored for control of tobacco powdery mildew and the systemic resistance mechanisms involved. The results of the study showed that endophyte YN201732 has antagonistic effects on 12 pathogenic fungi. The fermentation broth can inhibit the spore germination and has good control effect on tobacco powdery mildew in the greenhouse. The control effect of the protective test and the therapeutic test at 14 days reached up to 71.09% and 86.11%, respectively. Moreover, endophyte YN201732 can also promote seed germination and seedling plant growth, and improve plant photosynthesis. Compare with water treatment, the root length, plant height, maximum leaf length, leaf width, fresh weight, dry weight, chlorophyll and net photosynthetic rate of tobacco seedlings increased by 32.27%, 86.67%, 154.92%, 107.25%, 664.71%, 207.69%, 150.33%, and 240%, respectively. Additionally, the expression of NPR1 in tobacco leaves was stable when inoculated with YN201732 at different time points, while the PDF1.2 gene was up-regulated rapidly, suggesting that jasmonic acid/ethylene signaling pathway of plant might be induced, which enhance plant resistance to powdery mildew. The chit and PPO...

Source: Biological Control - November 29, 2019 Category: Biology Source Type: research