Site-directed RNA editing (SDRE): Off-target effects and their countermeasures

Publication date: Available online 27 November 2019Source: Journal of Genetics and GenomicsAuthor(s): Shaoshuai Mao, Yajing Liu, Shisheng Huang, Xingxu Huang, Tian ChiAbstractSite-directed RNA editing (SDRE) is invaluable to basic research and medicine, and has emerged as a new frontier in genome editing. The past few years have witnessed a surge of interest in SDRE, with SDRE tools emerging at a breathtaking pace. However, off-target effects of SDRE remain a tough problem, which constitutes a major hurdle to their clinical applications. Here we discuss the diverse strategies for combating off-target editing, drawing lessons from the published studies as well as our ongoing research. Overall, SDRE is still at its infancy, with significant challenges and exciting opportunities ahead.

Source: Journal of Genetics and Genomics - November 28, 2019 Category: Genetics & Stem Cells Source Type: research