Rapid Tuberculosis Diagnosis Using Reporter Enzyme Fluorescence Mycobacteriology and Aerobic Actinomycetes

We report the first evaluation of clinical specimens with REFtb assays in comparison to the gold standards for tuberculosis diagnosis, culture and smear microscopy. REFtb assays allowed diagnosis of 160 patients from 16 different countries with a sensitivity of 89% for smear-positive, culture-positive samples and 88% for smear-negative, culture-positive samples with a specificity of 82%. The negative predictive value of REFtb for tuberculosis infection is 93%, and the positive predictive value is 79%. Overall, these data point toward the need for larger accuracy studies by third parties using a commercially available REFtb kit to determine whether incorporation of REFtb into the clinical toolbox for suspected tuberculosis patients would improve case identification. If results similar to our own can be obtained by all diagnostic laboratories, REFtb would allow proper treatment of more than 85% of patients that would be missed during their initial visit to a clinic using current diagnostic strategies, reducing the potential for further spread of disease.
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Mycobacteriology and Aerobic Actinomycetes Source Type: research

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Abstract A persistently high burden of tuberculosis (TB) and low cure rates in South Africa call for frequent assessment of the effectiveness of the TB programme. We conducted a retrospective cohort study to evaluate treatment outcomes and associated factors among new TB patients taking standard regimen 1 TB treatment during 2010 in Pretoria, using the World Health Organization's six treatment outcomes classification. The 85 participants (of whom 59% were female) had co-infection with HIV in 70%. A total of 52% completed treatment, but only 15% were officially cured; 13% died and only 35% had an end-of-treatment s...
Source: Tropical Doctor - Category: Tropical Medicine Authors: Tags: Trop Doct Source Type: research
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Source: Journal of Infection - Category: Infectious Diseases Authors: Source Type: research
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Source: Molecular Biology Reports - Category: Molecular Biology Authors: Tags: Mol Biol Rep Source Type: research
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Source: The American Journal of Tropical Medicine and Hygiene - Category: Tropical Medicine Authors: Tags: Am J Trop Med Hyg Source Type: research
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Source: Spine - Category: Orthopaedics Tags: CLINICAL CASE SERIES Source Type: research
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Source: Military Medicine - Category: International Medicine & Public Health Tags: Mil Med Source Type: research
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Source: International Journal of Infectious Diseases - Category: Infectious Diseases Authors: Source Type: research
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Source: Pan American Journal of Public Health - Category: International Medicine & Public Health Tags: Rev Panam Salud Publica Source Type: research
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Source: Lasers in Medical Science - Category: Laser Surgery Source Type: research
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Source: Lung India - Category: Respiratory Medicine Authors: Source Type: research
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