A Novel Colorimetric Fluorescent Probe for Fe3+ Based on Tetraphenylethylene-rhodamine

Publication date: November 2019Source: Chinese Journal of Analytical Chemistry, Volume 47, Issue 11Author(s): Jia-Min CHEN, Jing ZENG, Zhen ZHANG, Abudu-Rexit ABULIKEMUAbstractA novel tetraphenylethylene-rhodamine-based fluorescent probe amide compound L was synthesized and its sensing properties of metal ions were investigated. It exhibited high sensitivity and high selectivity toward Fe3+ compared with other common metal ions in EtOH-H2O (50 μM, 3:2, V/V, pH 7.0). A fluorescence quenching ratio of 92% at 482 nm and 254-fold enhancement of fluorescence intensity at 582 nm were achieved upon the addition of 4.0 equiv. Fe3+. Under optimal experimental conditions, the fluorescence intensity of probe exhibited a dynamic response range for Fe3+ from 2.0 × 10−5 M to 1.5 × 10−4 M at 482 nm and 3.0 × 10−5 M to 1.5 × 10−4 M at 582 nm, respectively, with a detection limit of 1.56 × 10−7 M. Meanwhile, the binding ratio of L and Fe3+ was confirmed to be 1:1 by electrospray ionization-mass spectrometry (ESI-MS) analysis of L-Fe3+ complex and Benesi-Hildebrand method. According to 1H-nuclear magnetic resonance (NMR) titration and scanning electron microscopy results, the coordination mechanism of probe L with Fe3+ was further elucidated. The binding of Fe3+ to L not only led to the aggregation state dispersed and fluorescence quenching at 482 nm, but also induced spirolactum ring opening of rhodamine and aroused a 254 times fluorescence enhancement at 582 nm together with ...
Source: Chinese Journal of Analytical Chemistry - Category: Chemistry Source Type: research