Development of an EvaGreen based real-time RT-PCR assay for rapid detection, quantitation and diagnosis of goose calicivirus

In this study, an EvaGreen based fluorescence quantitative real-time RT-PCR assay was developed and optimized for the detection of GCVs. The assay sensitively detected GCV RNA template with a good linear standard curve We also demonstrated the specificity and reproducibility of the detection method for GCVs. Thus, the method developed in this study will benefit the investigation of possible sporadic outbreaks of CV infections in geese, as well as epidemiological and etiological studies of GCVs.
Source: Molecular and Cellular Probes - Category: Molecular Biology Source Type: research

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rvé Poulet Feline calicivirus (FCV) is a widespread and highly prevalent pathogen of domestic cats, responsible for mild upper respiratory tract disease. Outbreaks of severe virulent systemic disease (VSD) associated with FCV infection have been reported worldwide. VSD FCV strains have a broader tropism and cause a systemic vascular compromise. Despite clear differences in the pathogenesis of VSD and oral respiratory infections, attempts to identify specific molecular markers of VSD strains on the major capsid protein VP1 have failed. Region E of VP1 is responsible for the interaction with the cell receptor Junc...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research
ann-Lehmann Feline calicivirus (FCV) can cause painful oral ulcerations, salivation, gingivitis/stomatitis, fever and depression in infected cats; highly virulent virus variants can lead to fatal epizootic outbreaks. Viral transmission occurs directly or indirectly via fomites. The aim of this study was to investigate the presence and viability of FCV in the environment after sequential oronasal infections of specified pathogen-free cats with two FCV field strains in a research facility. Replicating virus was detected in saliva swabs from all ten cats after the first and in four out of ten cats after the second FCV exp...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research
Publication date: Available online 16 July 2019Source: Veterinary MicrobiologyAuthor(s): L. Camacho-Sillero, J. Caballero-Gómez, F. Gómez-Guillamón, A. Martínez-Padilla, M. Agüero, E. San Miguel, I. Zorrilla, E. Rayas, V. Talavera, I. García-BocanegraAbstractRabbit hemorrhagic disease (RHD) is a highly infectious disease in European rabbits (Oryctolagus cuniculus), caused by a virus belonging to the genus Lagovirus (RHDV; family Caliciviridae). In 2010, a new serotype RHDV (RHDV2/RHDVb, currently designated GI.2) emerged in France, affecting both domestic, even those vaccinated for t...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
ConclusionThis compound is readily accessible in bulk quantities, is inexpensive, and has a long history of human consumption, which endorses the idea for further assessment as an antiviral therapeutic. This work also suggests that a more exhaustive assessment of natural product libraries against EBOV and other viruses is warranted to improve our ability to identify compounds that are so distinct from FDA approved drugs.
Source: Pharmaceutical Research - Category: Drugs & Pharmacology Source Type: research
ConclusionThis study suggests that norovirus can survive being ejected even within small far-reaching droplets at concentrations capable of eliciting infection. Such droplets could easily go unnoticed and be overlooked during cleaning, adding to the challenge of controlling norovirus outbreaks.
Source: Journal of Hospital Infection - Category: Infectious Diseases Source Type: research
ConclusionThis study suggests that Norovirus can survive being ejected even within small far-reaching droplets at concentrations capable of eliciting infection. Such droplets could easily go un-noticed and be overlooked during cleaning, adding to the challenge of controlling Norovirus outbreaks.
Source: Journal of Hospital Infection - Category: Infectious Diseases Source Type: research
Romalde Viral agents of human gastroenteritis affect people of all ages across the globe. As a mainly self-limiting disease, it is difficult to evaluate the real prevalence of etiological agents circulating in each region. Many of the analyzed outbreaks are caused by viruses of the family Caliciviridae, especially the genus Norovirus (NoV). Most studies have focused on other enteric viruses, leaving sapovirus (SaV) underestimated as an important emerging human threat. This one-year study analyzed clinical samples from hospital outpatients with acute gastroenteritis in Spain, with the aim of revealing the importance of...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research
e; MJ Abstract Enteric viruses have been recognized as the leading cause of non-bacterial gastroenteritis and hepatitis outbreaks around the world. Understanding their prevalence and persistence in the environment is important for the effective control of these infections. The aim of this study was to develop an efficient recovery procedure for viral infectious particles from agricultural soils. Samples (25 g) of soil (black earth soil, loamy soil, and sandy soil) were spiked with murine norovirus (MNV) and feline calicivirus (FCV), mixed with five different buffers and viral genetic material was extracted by 3 ...
Source: Journal of Virological Methods - Category: Virology Authors: Tags: J Virol Methods Source Type: research
Norovirus is a small, non-enveloped, positive stranded RNA virus in the Caliciviridae family. Infection results in diarrhoea, vomiting, abdominal pain and fever lasting 1-3 days, although only 10-12% of individuals seek medical attention [1]. Norovirus is the most common cause of acute gastroenteritis worldwide and causes outbreaks in hospitals, nursing homes, cruise ships, and other closed settings [2 –5]. There are an estimated 19-21 million norovirus cases in the United States every year [6]. Outbreaks of norovirus not only confer significant morbidity but have also been shown to increase all-cause mortality, part...
Source: Journal of Clinical Virology - Category: Virology Authors: Source Type: research
Four of eleven affected dogs died despite aggressive treatment during a 2015 focal outbreak of hemorrhagic gastroenteritis following a stay in a pet housing facility. Routine diagnostic investigations failed to identify a specific cause. Virus isolation from fresh necropsy tissues yielded a calicivirus with sequence homology to a vesivirus within the group colloquially known as the vesivirus 2117 strains that were originally identified as contaminants in CHO cell bioreactors. In situ hybridization and reverse transcription-PCR assays of tissues from the four deceased dogs confirmed the presence of canine vesivirus (CaVV) n...
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Clinical Veterinary Microbiology Source Type: research
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