Rev1 wbdR tagged vaccines against Brucella ovis

AbstractSheep brucellosis is a worldwide extended disease caused byB. melitensis andB. ovis, two species respectively carrying smooth or rough lipopolysaccharide. VaccineB. melitensis Rev1 is used againstB. melitensis andB. ovis but induces an anti-smooth-lipopolysaccharide response interfering withB. melitensis serodiagnosis, which precludes its use againstB. ovis whereB. melitensis is absent. In mice, Rev1 deleted inwbkC (Brucella lipopolysaccharide formyl-transferase) and carryingwbdR (E. coli acetyl-transferase) triggered antibodies that could be differentiated from those evoked by wild-type strains, was comparatively attenuated and protected againstB. ovis, suggesting its potential as aB. ovis vaccine.
Source: Veterinary Research - Category: Veterinary Research Source Type: research

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In this study we evaluated the role of the FlgJ, a protein, presumably a peptidoglycan hydrolase involved in flagellum formation and in the virulence of B. abortus strain 2308. B. abortus 2308 ΔflgJ mutant and complemented strains were constructed to study the function of the FlgJ protein in the context of the virulence of this pathogen in in vitro and in vivo assays. The results showed that the elimination of the flgJ gene delays the growth rate of B. abortus in culture, reduces its intracellular survival capacity in professional and non-professional phagocytic cells, rendering it unable to escape from the endocytic...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
In conclusion, mAbs against Omp25 are precious reagents for detection of Brucellae in clinical samples with various immunoassays. mAb 6C12-based FCM could be potentially used for the monitoring of therapeutic efficacy for brucellosis in clinical practice.
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
In conclusion, RPA is a useful and convenient field and point of care test for brucellosis.
Source: Molecular and Cellular Probes - Category: Molecular Biology Source Type: research
In conclusion, we unraveled the secretome of G. adiacens, an oral bacterium well-documented in infective endocarditis, but also recently shown to be involved in oral infections. Importantly, the secretome of G. adiacens comprised of a large number of putative virulence factors. Of particular importance is the finding that the G. adiacens secretome comprised of a number of “moonlighting” proteins, which in other species are shown to enhance bacterial colonization and virulence through their multifunctional roles (Pavkova et al., 2017; Graf et al., 2019). Thus, our results provide a basis for investigating the ro...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
Abstract Zoonoses such as Brucella, Mycobacterium bovis, Campylobacter, Salmonella, Escherichia coli, Listeria, etc. are spread by ingestion of contaminated unpasteurised milk products (from infected cattle) and result in infectious outbreaks, hospitalisations and mortality. In Southeast Asian countries, raw goat's milk is sometimes used for dengue infection-associated thrombocytopenia; however, evidence to support this sociocultural practice is lacking. A peak incidence of brucellosis cases may occur after a dengue season in communities where this practice is prevalent. PMID: 30895885 [PubMed - as supplied by publisher]
Source: Tropical Doctor - Category: Tropical Medicine Authors: Tags: Trop Doct Source Type: research
Since August 2017, CDC has confirmed three cases of brucellosis attributed to Brucella abortus cattle vaccine strain RB51. Each case was associated with consumption of domestically acquired unpasteurized (raw) milk products.
Source: CDC Morbidity and Mortality Weekly Report - Category: American Health Tags: Birth Defects Campylobacteriosis E. coli Infection Emergency Preparedness & Response Lead Poisoning MMWR Morbidity Mortality Weekly Report Outbreaks Public Health Raw Milk (Unpasteurized Milk) Water Treatment Brucella Infection Source Type: news
This study was designed with the aim to construct the DNA based vaccine of gene encoding antigenic surface protein (BCSP31). For this purpose, the BCSP31 gene was amplified, purified and ligated in pTZ57 R/T (cloning vector). Dubbed BCSP31-pTZ57 R/T vector was transformed into competent cells (DH5α). After plasmid extraction, the plasmid and pET-28a vector was restricted with EcoRI and BamHI. Again, ligation was done and dubbed pET-28a-BCSP31 transformed into E. coli (BL21). After expression, the protein was purified and used for evaluation of immunogenic response. The protective and immunogenic efficacy of the v...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
CONCLUSIONS: This report describes an emergent case and the response to two consecutive outbreaks of occupational brucellosis among workers in a pharmaceutical manufacturing enterprise. The hierarchical control strategy used in this case may prevent new outbreaks, and several lessons can be learned from this event. PMID: 30068856 [PubMed - as supplied by publisher]
Source: Journal of Occupational Health - Category: Occupational Health Tags: J Occup Health Source Type: research
Summary The natural fecundity of suids, great ability to adapt to new habitats and desire for local hunting opportunities leading to translocation of feral pigs to regions where they are not yet established have all been instrumental in the home range expansion of feral swine. Feral swine populations in the United States continue to expand, wreaking havoc on agricultural lands, further compromising threatened and endangered species, and posing a microbiological threat to humans, domestic livestock and companion animals. This manuscript thoroughly reviews zoonotic diseases of concern including brucellosis, bovine tuberculos...
Source: Transboundary and Emerging Diseases - Category: Veterinary Research Authors: Tags: REVIEW ARTICLE Source Type: research
In this study, it was aimed to identify the Brucella strains to the species level isolated from blood cultures, and to determine the rate of antimicrobial susceptibility against eleven antibacterial agents. A total of 106 Brucella spp. strains were included in the study, which were isolated from blood cultures in University of Health Sciences, Konya Training and Research Hospital, Medical Microbiology Laboratory between January 2011 and June 2013. Identification of the isolated strains were mainly based on conventional methods. In vitro antibacterial susceptibilities of azithromycin, ciprofloxacin, doxycycline, gentamicin,...
Source: Mikrobiyoloji Bulteni - Category: Microbiology Tags: Mikrobiyol Bul Source Type: research
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