Rev1 wbdR tagged vaccines against Brucella ovis

AbstractSheep brucellosis is a worldwide extended disease caused byB. melitensis andB. ovis, two species respectively carrying smooth or rough lipopolysaccharide. VaccineB. melitensis Rev1 is used againstB. melitensis andB. ovis but induces an anti-smooth-lipopolysaccharide response interfering withB. melitensis serodiagnosis, which precludes its use againstB. ovis whereB. melitensis is absent. In mice, Rev1 deleted inwbkC (Brucella lipopolysaccharide formyl-transferase) and carryingwbdR (E. coli acetyl-transferase) triggered antibodies that could be differentiated from those evoked by wild-type strains, was comparatively attenuated and protected againstB. ovis, suggesting its potential as aB. ovis vaccine.
Source: Veterinary Research - Category: Veterinary Research Source Type: research