Lipopolysaccharide Stress Induces Cryptic Exon Splice Variants of the Human Glucocorticoid Receptor

Glucocorticoids are widely used in the treatment of numerous inflammatory conditions, including sepsis. Unfortunately, patient response to glucocorticoid therapy can be inconsistent. Variations in the human glucocorticoid receptor (hGR) may contribute to the differential patient response. We screened for hGR variants in the buffy coats of burn patients and peripheral blood mononuclear cells (PBMCs) treated with lipopolysaccharide. Three novel splice variants containing cryptic exons were upregulated in the PBMCs after lipopolysaccharide exposure at 3 and 13 h with the greatest observed expression at 3 h. Luciferase assays revealed that two of the isoforms had no significant activity in comparison with the reference hGR when stimulated with hydrocortisone. The third isoform had an augmented response that was greater than the reference hGR at a high cortisol dose. This shows that PBMCs are able to produce variant hGR isoforms in response to stress. Furthermore, lipopolysaccharide stress appears to induce these hGR variants, potentially by influencing mRNA splicing. In the future, identifying hGR expression profiles may be a key component in individually tailoring a patient's treatment to sepsis and injury.
Source: Shock - Category: Emergency Medicine Tags: Clinical Science Aspects Source Type: research

Related Links:

Scientists from Loughborough University found people ate 65 fewer calories per meal and 200 less each day when exposed to the messages on the packaging of their food.
Source: the Mail online | Health - Category: Consumer Health News Source Type: news
Saying how far consumers need to walk to burn off the calories could change eating habitsLabelling food and drinks with how much walking or running is needed to burn them off could help tackle the obesity crisis, researchers say.While all packaged food must display certain nutritional information, such as calorie content,there is limited evidence that the approach changes what people buy or eat. Meanwhile,waistlines continue to expand.Continue reading...
Source: Guardian Unlimited Science - Category: Science Authors: Tags: Obesity Food Health Society UK news Science Nutrition Source Type: news
ConclusionThese data suggest that a combined 2-h infusion with a higher dosage of meropenem, including a loading dose, may be successful to achieve effective PK parameters.
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
Boston-headquartered Orbita, a healthcare innovation company offering conversational AI platform and virtual health assistant solutions, has released the OrbitaASSIST, a voice-powered, AI-driven bedside virtual assistant. Patients use a smart speaker...
Source: Medgadget - Category: Medical Devices Authors: Tags: Exclusive Informatics Source Type: blogs
Condition:   Burns Intervention:   Other: protocol of management of major burn in intensive care unit Sponsor:   Assiut University Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Objectives: Frailty and disability are commonly found in Late Life Depression (LLD) and have been associated with increased depression severity, health comorbidities and mortality. Additionally, physical frailty has been associated with suicide in l...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Age: Elder Adults Source Type: news
Chitosan (CS) and phosphorylated chitosan (PCS) were deposited onto the polyamide 66 (PA66) fabric surfaces along with poly-acrylate sodium (PAS) via 'one pot' and layer by layer (LbL) assembly methods in preparing flame retardant coatings. Subsequently, t...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Burns, Electricity, Explosions, Fire, Scalds Source Type: news
INTRODUCTION: Choking agent exposure, among them chlorine gas, occurs in household or industrial accidents, chemical warfare and terrorist attacks. AIMS: Review of published animal and human data regarding the history, pathophysiology, clinical effects and...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Burns, Electricity, Explosions, Fire, Scalds Source Type: news
Chromium is ubiquitous in the environment, found in chrome plated metals, tanned shoe leather, cement, detergents.1 Following cutaneous exposure chromium can cause adverse reactions, mainly allergic contact dermatitis (ACD) and an irritant react...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Burns, Electricity, Explosions, Fire, Scalds Source Type: news
Three vehicles with trunks containing pig (Sus scrofa domesticus Erxleben) carcasses which had been allowed to decompose for 30 days, were set alight in controlled burns to determine whether forensically valuable insect evidence could still be recovered. E...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Burns, Electricity, Explosions, Fire, Scalds Source Type: news
More News: Burns | Emergency Medicine | Hydrocortisone | Science