The α1-adrenoceptor-mediated human hyperplastic prostate cells proliferation is impaired by EGF receptor inhibition

Publication date: Available online 12 November 2019Source: Life SciencesAuthor(s): Jessica Barbosa Nascimento-Viana, Rocío Alcántara-Hernández, Eliane Oliveira-Barros, Luiza A. Castello Branco, Priscilla R. Feijó, Luiz Antonio Soares Romeiro, Luiz Eurico Nasciutti, François Noël, J. Adolfo García-Sáinz, Claudia Lucia Martins SilvaAbstractBenign prostatic hyperplasia (BPH) is an aging related and progressive disease linked to an up-regulation of α1-adrenoceptors. The participation of EGF receptors (EGFR) in the GPCRs´ signalosome has been described but so far data about the contribution of these receptors to prostatic stromal hyperplasia are scanty. We isolated and cultured vimentin-positive prostate stromal cells obtained from BPH patients. According to intracellular Ca2+ measurements, cell proliferation and Western blotting assays, these cultured hyperplastic stromal cells express functional α1-adrenoceptors and EGFR, and proliferate in response to the α1-adrenoceptor agonist phenylephrine. Interestingly, in these cells the inhibition of EGFR signaling with GM6001, CRM197, AG1478 or PD98059 was associated with full blockage of α1-adrenoceptor-mediated cell proliferation, while cell treatment with each inhibitor alone did not alter basal cell growth. Moreover, the co-incubation of AG1478 (EGFR inhibitor) with α1A/α1D-adrenoceptor antagonists showed no additive inhibitory effect. These findings highlight a putative role of EGFR signaling to α1-adrenoceptor-medi...

Source: Life Sciences - November 13, 2019 Category: Biology Source Type: research