Time and dose-dependent effects of viscum album quercus on rabbit spermatozoa motility and viability in vitro.

Time and dose-dependent effects of viscum album quercus on rabbit spermatozoa motility and viability in vitro.
Physiol Res. 2019 Oct 25;
Authors: Halo M, Massanyi P, Gren A, Lasak A, Slanina T, Ondruska L, Muchacka R, Galbavy D, Ivanic P, Schneir ER, Formicki G
Abstract
The target of this study was to evaluate the effect of extract of the European mistletoe - Viscum album quercus L. on spermatozoa motility and viability in vitro. The CASA system was used to determine the spermatozoa motility parameters at different time intervals (0, 1, 2 and 3 h) and spermatozoa viability was determined in five different doses of Viscum album quercus L [10 (QA), 6.6 (QB), 3.3 (QC), 2.5 (QD) and 2 (QE) mg/ml]. Results in experimental groups detected a significant deterioration on rabbit spermatozoa after 1, 2 and 3 hours, compared to the control. The initial total spermatozoa motility showed increased value for all doses of Viscum album quercus in comparison to control. After in vitro culture a dose-dependent decrease (QA: reduction of 69.7 %, QB: reduction of 40.9 %) was found. For the progressive spermatozoa most significant decrease (86.8 % for QA vs. 48.5 % for QB) was detected compared to the control after 3 hours of culture. Spermatozoa viability (MTT test) was decreased in all experiment groups at the end of experiment, but the differences were not significant. Significant alterations of membrane integrity were found in groups with the highest...

Source: Physiological Research - October 24, 2019 Category: Physiology Authors: Halo M, Massanyi P, Gren A, Lasak A, Slanina T, Ondruska L, Muchacka R, Galbavy D, Ivanic P, Schneir ER, Formicki G Tags: Physiol Res Source Type: research