Selective cross-linking behavior of oligodeoxyribonucleotides containing 2'-O-[N-(4,5',8-trimethylpsoralen-4'-ylmethylcarbamoyl)]adenosine to mutant H-ras DNA.

Selective cross-linking behavior of oligodeoxyribonucleotides containing 2'-O-[N-(4,5',8-trimethylpsoralen-4'-ylmethylcarbamoyl)]adenosine to mutant H-ras DNA.
Nucleosides Nucleotides Nucleic Acids. 2019 Oct 24;:1-12
Authors: Yamayoshi A, Higuchi M, Sakai Y, Kobori A, Yamamoto T, Shibata T, Murakami A
Abstract
Point mutations are well characterized activators of oncogenes but are often indistinguishable using common gene technologies. In general, the precise sites of point-mutated oncogenes are difficult to distinguish under physiological conditions primarily because single nucleotide mismatch do not affect the annealing temperatures of DNA probes sufficiently. To address this limitation, we developed photo-responsive oligodeoxyribonucleotides containing 2'-O-[N-(4,5',8-trimethylpsoralen-4'-ylmethylcarbamoyl)]adenosine (Ps-amd-Oligo), which can be used to selectively manipulate and identify genes with point mutations. Here we present time course analyses of the photo-cross-linking efficiency of Ps-amd-Oligo with DNA and RNA and show promising selectivity for the oncogene H-ras.
PMID: 31645189 [PubMed - as supplied by publisher]

Source: Nucleosides, Nucleotides and Nucleic Acids - October 26, 2019 Category: Biochemistry Tags: Nucleosides Nucleotides Nucleic Acids Source Type: research