Evaluation of str profiles of single telogen hairs using probabilistic methods

Full STR profiles can rarely be obtained from single telogen hairs due to the minute amount and compromised integrity of their DNA. We developed a next generation sequencing (NGS)-based STR assay, called maSTR (mini-amplicon STR) assay that is particularly suited for degraded DNA. The maSTR assay yielded higher allele recoveries than capillary electrophoresis (CE)-based STR analysis from DNA from single telogen hairs, however displayed a higher number of allelic drop-ins. To calculate likelihood ratios for assignment to correct donors, profiles composed by consensus or composite interpretation were subjected to fully or semicontinuous probabilistic methods using the GenoProof Mixture 3 software.

Source: Forensic Science International: Genetics Supplement Series - October 20, 2019 Category: Forensic Medicine Authors: Sarah Aurora He ß, Richard Jäger Source Type: research