White blood cell labeling with Technetium-99m (99mTc) using red blood cell extracellular vesicles-mimetics

Publication date: Available online 20 October 2019Source: Blood Cells, Molecules, and DiseasesAuthor(s): Seung Hyun Son, Ji Min Oh, Prakash Gangadaran, Hyun Dong Ji, Ho Won Lee, Ramya Lakshmi Rajendran, Se Hwan Baek, Arunnehru Gopal, Senthilkumar Kalimuthu, Shin Young Jeong, Sang-Woo Lee, Jaetae Lee, Byeong-Cheol AhnAbstractBackgroundExtracellular vesicles, have gained increasing attention for their application in drug delivery. Here, we developed a novel method for radiolabeling WBCs with 99mTc using RBC-derived extracellular vesicles -mimetics (EVMs), and monitored in vivo inflammation tracking of 99mTc-WBC using gamma camera in acute inflammation mouse model.MethodsEngineered EVMs from RBCs were produced by a one-step extrusion method. RBC-EVMs were analyzed by NTA and TEM. Cells were labeled with 99mTc by using 99mTc-RBC-EVMs. Inflammation mice model was prepared and confirmed by 18F-FDG PET/CT. 99mTc-WBCs were injected in mice, and their biodistribution was analyzed by gamma camera.FindingThe radiochemical purity of 99mTc-RBC-EVMs was 100%. The 99mTc-labeling did't affect the size and morphology. The 99mTc in the cytoplasm of RBC-EVMs was successfully confirmed by high angle annular dark field STEM (scanning transmission electron microscope). Cells were successfully labeled with 99mTc using 99mTc-RBC-EVMs, and the counts per minute was increased in dose- and time-dependent manners. The 18F-FDG PET/CT images confirmed establishment of acute inflammation (left mouse foot)....

Source: Blood Cells, Molecules, and Diseases - October 21, 2019 Category: Hematology Source Type: research