A statistical model for activation of Factor C by binding to LPS aggregates.

A statistical model for activation of Factor C by binding to LPS aggregates.
Eur Biophys J. 2019 Oct 19;:
Authors: Miyagawa Y, Kikuchi K, Tsuchiya M, Adachi S
Abstract
Published data on Factor C activity at various LPS and Lipid A concentrations (Nakamura et al. in Eur J Biochem 176:89, 1988; Kobayashi et al. in J Biol Chem 37:25987, 2014) were rearranged to show that Factor C exhibited its maximum activity at a specific concentration of LPS. A statistical model was proposed for examining whether a single LPS molecule binding activates Factor C (monomeric activation) or dimerization of Factor C is necessary for the activation (dimeric activation). In the monomeric activation model the plots of the relative activity of Factor C against the molar ratio of LPS to Factor C were different from those in the published data. The plots in the dimeric activation model lie on a bell-shaped curve, whatever the Factor C concentration, matching the published data and indicating the appropriateness of that model. We suggest that Factor C is activated by multiple molecular interactions of Factor C with LPS aggregates on which it dimerises and that this explains why larger aggregates are less effective at activating Factor C than smaller ones.
PMID: 31630219 [PubMed - as supplied by publisher]

Source: European Biophysics Journal : EBJ - October 18, 2019 Category: Physics Authors: Miyagawa Y, Kikuchi K, Tsuchiya M, Adachi S Tags: Eur Biophys J Source Type: research