Volatile organic compound (VOC) emissions of CHO and T cells correlate to their expansion in bioreactors

Volatile organic compound (VOC) emissions were measured from Chinese Hamster Ovary (CHO) cell and T
cell bioreactor gas exhaust lines with the goal of non-invasively metabolically profiling the
expansion process. Measurements of cellular ‘breath’ were made directly from the gas exhaust lines
using polydimethylsiloxane (PDMS)-coated magnetic stir bars, which underwent subsequent thermal
desorption-gas chromatography-mass spectrometry (TD –GC–MS) analysis. Baseline VOC profiles were
observed from bioreactors filled with only liquid media. After inoculation, unique VOC profiles
correlated to cell expansion over the course of 8 d. Partial least squares (PLS) regression models
were built to predict cell culture density based on VOC profiles of CHO and T cells ( R 2  = 0.671
and R 2  = 0.769, respectively, based on a validation data set). T cell runs resulted in 47 compounds
relevant to expansion while CHO cell runs resulted in 45 compounds; the 20 most...

Source: Journal of Breath Research - October 19, 2019 Category: Respiratory Medicine Authors: Mitchell M McCartney, Mei S Yamaguchi, Paul A Bowles, Yarden S Gratch, Rohin K Iyer, Angela L Linderholm, Susan E Ebeler, Nicholas J Kenyon, Michael Schivo, Richart W Harper, Paul Goodwin and Cristina E Davis Source Type: research