Generation of a genetically modified human embryonic stem cells expressing fluorescence tagged ATOX1

Publication date: Available online 16 October 2019Source: Stem Cell ResearchAuthor(s): Meng-Hsuan Wen, Xihong Xie, Jian Tub, Dung-Fang Lee, Tai-Yen ChenAbstractATOX1 is a copper chaperone involved in intracellular copper homeostasis, cell proliferation, and tumor progression. To investigate the physiologically relevant molecular mechanism of ATOX1 by using imaging-based approaches, we genetically modified ATOX1 in H1 hESCs to express mCherry-ATOX1 fusion protein under endogenous regulatory machinery. The fluorescence engineered hESC clone maintains characteristic stem cell features and can differentiate to all three germ layers, serving as a unique tool to dissect the role of ATOX1 in various cellular processes.
Source: Stem Cell Research - Category: Stem Cells Source Type: research