Direct monitoring of equilibrium protein folding-unfolding by atomic force microscopy: pushing the limit.

We report the direct observation of equilibrium folding-unfolding dynamics of a mechanically labile, three helix bundle protein GA using a commercial atomic force microscope (AFM). Our study extends the capability of commercial AFMs towards studying protein unfolding/refolding at equilibrium as well as increasingly complex biomolecular systems in the context of biological function.
PMID: 31608905 [PubMed - as supplied by publisher]

Source: Chemical Communications - October 13, 2019 Category: Chemistry Authors: Xiao A, Li H Tags: Chem Commun (Camb) Source Type: research