A novel electrochemiluminescence biosensor: inorganic-organic nanocomposite and ZnCo2O4 as the efficient emitter and accelerator

Publication date: Available online 5 October 2019Source: Sensors and Actuators B: ChemicalAuthor(s): Yang Li, Yanxin Chu, Yuanfang Li, Cheng Ma, Lingling LiAbstractThe detection of specific DNA sequences provides a powerful tool for early diagnosis and prevention of disease. Herein, an enzyme-free electrochemiluminescence (ECL) DNA biosensor was developed by coupling inorganic-organic nanocomposite as the luminophore and ZnCo2O4 as the efficient accelerator. ZnCo2O4 nanoparticles (ZCO NPs), derived from Co and Zn based bimetallic zeolite imidazole framework, was used as the ECL substrate due to their large surface area and excellent catalytic effect. AgInS2-ZnS quantum dots (ZAIS) were covalently linked with meso-tetra(4-carboxyphenyl)-porphine (TCPP) to form ZAIS/TCPP inorganic-organic nanocomposites, which were used for the first time as the cathodic ECL luminophore with the coreactant of K2S2O8. Detection of target DNA (tDNA) about chronic myeloid leukemia (CML) was achieved based on dual-signal amplification via cascade toehold-mediated strand displacement reaction (TMSDR). On the basis of experimental results, a limit of detection (LOD) of 200 aM was achieved in the range of 0.5 fM to 1 nM, which showed the excellent performance of this biosensor. This work not only indicates that inorganic-organic nanocomposites as the ECL luminophore is a feasible strategy for efficient emission, but also expands the application of nanomaterials derived from metal organic framewo...
Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research