Development of an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of ringworm infection in cattle.

Development of an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of ringworm infection in cattle. Clin Vaccine Immunol. 2013 Jun 5; Authors: Bagut ET, Cambier L, Heinen MP, Cozma V, Monod M, Mignon B Abstract The aim of this study was to develop an in-house ELISA for the serological diagnosis of ringworm infection in cattle. We used available recombinant forms of Trichophyton rubrum dipeptidyl peptidase V (TruDppV) and leucin aminopeptidase 2 (TruLap2), which are 98% identical to Trichophyton verrucosum orthologues. Field serum samples from 135 cattle with ringworm infection as confirmed by direct microscopy, fluorescence microscopy and PCR, and from 55 cattle without any apparent skin lesions or history of ringworm that served as negative controls were used. Sensitivity, specificity, and positive and negative predictive values were determined to evaluate the diagnostic value. Overall, the ELISAs based on recombinant TruDppV and TruLap2 discriminated well between infected animals and healthy controls. A highly significant difference (P < 0.0001, Mann-Whitney U test) was noted between optical density values obtained when testing sera from infected versus control cattle. The ELISA developed for the detection of specific antibodies against DppV gave 89.6% sensitivity, 92.7% specificity, 96.8% positive predictive value and 78.4% negative predictive value. The recombinant TruLap2-based ELISA displayed 88.1% sensitivity, 90.9% sp...
Source: Clinical and Vaccine Immunology - Category: Allergy & Immunology Authors: Tags: Clin Vaccine Immunol Source Type: research