Production and characterization of multifacet exopolysaccharide from an agricultural isolate, Bacillus subtilis

This study aims to explore the fermentative production and physicochemical properties of an exopolysaccharide (EPS) produced from agricultural isolate,Bacillus subtilis S1 in submerged culture. The structural characterization (UV ‐Visible, FT‐IR spectroscopy, and13C NMR spectrometry) revealed that the EPS is an acidic heteropolymer consisting of glucose, glucuronic acid, pyruvic acid, and succinic acid. The non Newtonian shear thickening nature of EPS with a 1.55 ×107Da molecular weight is confirmed by rheology analysis. The extracted EPS was 61.3% amorphous with partial crystallinity (38.7 %) as confirmed by XRD analysis. The EPS shows two step decomposition and thermal stability up to 300◦C as confirmed by TGA and DSC analysis. The EPS has a small Z ‐average particle size (74.29 nm), high porosity (92.99 %), high water holding (92.39 %) and absorption capacity (1198 %). The biocompatible nature is confirmed by cytotoxic testing on the human keratinocytes cell line. The demonstrated unique characteristics ofBacillus EPS presents it as a choice of biomaterial for diverse applications.This article is protected by copyright. All rights reserved

Source: Biotechnology and Applied Biochemistry - September 19, 2019 Category: Biochemistry Authors: Bhavana V. Mohite, Sunil H. Koli, Jamatsing D. Rajput, Vikas S. Patil, Tarun Agarwal, Satish V. Patil Tags: Original Article Source Type: research