Defective Transcriptional Programming of Effector CD8 T Cells in Aged Mice Is Cell-Extrinsic and Can Be Corrected by Administration of IL-12 and IL-18

In response to infection with intracellular microorganisms, old mice mobilize decreased numbers of antigen-specific CD8 T cells with reduced expression of effector molecules and impaired cytolytic activity. Molecular mechanisms behind these defects and the cell-intrinsic (affecting naïve CD8 T cells themselves) vs. extrinsic, microenvironmental origin of such defects remain unclear. Using reciprocal transfer experiments of highly purified naïve T cells from adult and old transgenic OT-1 mice, we decisively show that the dominant effect is cell-extrinsic. Naïve adult OT-1 T cells failed to expand and terminally differentiate in the old organism infected with Listeria-OVA. This defect was preceded by blunted expression of the master transcription factor T-bet and impaired glycolytic switch when T cells are primed in the old organism. However, both old and adult naïve CD8 T cells proliferated and produced effector molecules to a similar extent when stimulated in vitro with polyclonal stimuli, as well as when transferred into adult recipients. Multiple inflammatory cytokines with direct effects on T cell effector differentiation were decreased in spleens of old animals, particularly IL-12 and IL-18. Of note, in vivo treatment of mice with IL-12 and IL-18 on days 4–6 of Listeria infection reconstituted cytotoxic T cell response of aged mice to the level of adult. Therefore, critical cytokine signals which are underproduced in the old priming environment c...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research

Related Links:

Authors: Ghersin I, Abu Alheija O, Azzam ZS, Nasser R PMID: 31599514 [PubMed - in process]
Source: The Israel Medical Association Journal - Category: General Medicine Tags: Isr Med Assoc J Source Type: research
Publication date: Available online 10 October 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Fariha Ibrahim, Syeda Bushra Zafar, Afsheen Aman, Shah Ali Ul Qader, Asma AnsariAbstractOver the last few decades, emerging cases of chemical additives and food borne pathogens have enforced the demand of natural antimicrobial compounds. Bacteriocins are one of the natural alternatives to the chemical food preservatives. The research on new and improved bacteriocins is in full swing due to some limitations of commercially available bacteriocins. The aim of the study is to improve the indigenously isolated strain ...
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research
The U. S. Department of Agriculture ’s Food Safety and Inspection Service (FSIS) has announced that Tip Top Poultry, Inc, is recalling some of its frozen cooked, diced or shredded ready-to-eat chicken products.
Source: Healthcare News - Category: Pharmaceuticals Authors: Source Type: news
Do you store chicken in your freezer? You might want to take a look at the label.
Source: - Health - Category: Consumer Health News Source Type: news
Publication date: January 2020Source: LWT, Volume 118Author(s): Yuanyuan Pan, Yan Zhang, Jun-Hu Cheng, Da-Wen SunAbstractEffects of ultrasound pretreatments and dielectric barrier discharge (DBD) cold plasma on the inactivation of Listeria monocytogenes (L. monocytogenes) under various growth temperatures were investigated, and membrane fatty acid profiles of L. monocytogenes were also determined. Fatty acids anteiso-C15:0, anteiso-C17:0 and C15:0 were the most abundant fatty acids at any given growth temperature (10, 25, 37 and 42 °C). Principal components analysis (PCA) and redundancy analysis (RDA) displayed that ...
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
Jumbo Foods Inc. of Mukilteo, WA has issued a voluntary recall of Tuscan Sun (TS) brand chicken salad sandwiches, because it has the potential to be contaminated with Listeria monocytogenes
Source: FDA Food Safety - Category: Food Science Authors: Source Type: alerts
A laboratory-based study testing 9 Listeria innocua strains independently and a cocktail of 11 Listeria monocytogenes strains was carried out. The aim was to identify suitable L. innocua strain(s) to model L. monocytogenes in inactivation experiments. Three separate inactivation procedures and a hurdle combination of the three were employed: thermal inactivation (55°C), UV-C irradiation (245 nm), and chemical sanitizer (TsunamiTM 100, a mixture of acetic acid, peroxyacetic acid, and hydrogen peroxide). The responses were strain dependent in the case of L. innocua with different strains responding differently to differe...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
During infection, bacterial pathogens successfully sense, respond and adapt to a myriad of harsh environments presented by the mammalian host. This exquisite level of adaptation requires a robust modulation of their physiological and metabolic features. Additionally, virulence determinants, which include host invasion, colonization and survival despite the host's immune responses and antimicrobial therapy, must be optimally orchestrated by the pathogen at all times during infection. This can only be achieved by tight coordination of gene expression. A large body of evidence implicate the prolific roles played by bacterial ...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
Lipari Foods has issued a voluntary recall of Premo Ham&Cheese Wedge Sandwiches and Fresh Grab Ham&Cheese Wedge Sandwiches due to potential contamination of Listeria monocytogenes
Source: FDA Food Safety - Category: Food Science Authors: Source Type: alerts
CNN's Elizabeth Cohen explains what listeria is and how you can avoid getting it.
Source: - Health - Category: Consumer Health News Source Type: news
More News: Allergy & Immunology | Environmental Health | Listeria