Application of chemometrics for detection and modeling of adulteration of fresh cow milk with reconstituted skim milk powder using voltammetric fingerpriting on a graphite/ SiO2 hybrid electrode.

Application of chemometrics for detection and modeling of adulteration of fresh cow milk with reconstituted skim milk powder using voltammetric fingerpriting on a graphite/ SiO2 hybrid electrode.
Talanta. 2020 Jan 01;206:120223
Authors: Nikolaou P, Deskoulidis E, Topoglidis E, Kakoulidou AT, Tsopelas F
Abstract
In the present work, an analytical approach for the voltammetric detection and prediction of adulteration of fresh cow milk with reconstituted skim milk powder is developed. After precipitation of milk proteins upon addition of ethanol and centrifugation, the supernatant liquid of the samples was analyzed by cyclic voltammetry on a novel graphite/SiO2 hybrid working electrode (GSiHE) using LiClO4 as electrolyte. Under these conditions, fresh milk samples gave broadened peaks/plateaus in both forward and backward potential scanning, attributed mainly to oxidases. Such peaks were not evident in the case of reconstituted skim milk powder samples due to inactivation of enzymes and breakdown of certain antioxidants caused by heat and pressure-treatments. The differences between fresh and reconstituted skim milk powder samples in their voltammetric profile were exploited for the detection of fresh milk adulteration by submitting voltammetric data to chemometrics. As datapoints, the differences between forward and backward current values, recorded at the same potentials, were determined and submitted to multivariate analysis. Princip...

Source: Talanta - September 15, 2019 Category: Chemistry Authors: Nikolaou P, Deskoulidis E, Topoglidis E, Kakoulidou AT, Tsopelas F Tags: Talanta Source Type: research