Genetic diversity between and within the natural populations of Garcinia indica (Thouars) Choisy: A high value medicinal plant from Northern Western Ghats of India using ISSR markers

Publication date: Available online 7 September 2019Source: Journal of Applied Research on Medicinal and Aromatic PlantsAuthor(s): Rutuja S. Palkar, Krishnan SellappanAbstractThe present study reveals genetic diversity between and within the natural populations of Garcinia indica which is an endemic and medicinally important tree species from the Western Ghats of India. During the study extensive field survey was carried out to different parts of the region and collected leaf samples from 11 different locations to assess genetic diversity between populations. To understand the genetic diversity within the population, leaf samples were collected from seven different locations (accessions) spread over the single large population at Amboli, Maharashtra. Out of 21 ISSR primers screened, 12 primers produced reproducible, clear banding pattern. A total of 127 bands amplified for the samples analyzed in between the populations, among them 119 were polymorphic and percent polymorphism varied from 80 to 100% per primer. Genetic diversity within the population shown a total of 85 amplified bands, out of which 74 were polymorphic and polymorphic percentages varied from 60 to 100% per primer. The other genetic diversity parameters revealed high diversity and differentiation in both between populations (percent polymorphism = 93.87%, h = 0.3196, na = 1.82, ne = 1.37, I = 0.41, Ht = 0.26 and GST = 0.81) and within population (percent polymorphism = 84.80%, hâ...
Source: Journal of Applied Research on Medicinal and Aromatic Plants - Category: Complementary Medicine Source Type: research