Nystatin complexation with β-cyclodextrin: Spectroscopic evaluation of inclusion by FT-Raman, photoacoustic spectroscopy, and 1H NMR

Publication date: 1 January 2020Source: Materials Chemistry and Physics, Volume 239Author(s): Aloisi Somer, João Ricardo Roik, Mauricio Aparecido Ribeiro, Amanda M. Urban, Amanda Schoeffel, Vanessa M. Urban, Paulo V. Farago, Lidiane Vizioli de Castro, Francielle Sato, Carlos Jacinto, Eliane Campesatto, Magna Suzana Alexandre Moreira, Andressa NovatskiAbstractThe inclusion complex of Nystatin (NYS) with β-cyclodextrin (β-CD) can increase the solubility of the antibiotic in aqueous media. The complexes were obtained by freeze-drying (FD) and spray-drying (SD) methods. The spectroscopic techniques used to analyze the complexes were FT-Raman, photoacoustic spectroscopy (PAS), and 1H NMR. FT-Raman allowed the identification of the interactions of β-CD with NYS molecule through the vibrational modes assigned to tetraene, heptaene and C=O. PAS confirms that the main part of interaction from NYS is heptaene. The 1H NMR showed that the hydrogens inside the cavity of β-CD interact with NYS. These techniques confirmed the complex formation and that the drying method affects the form of complexation: by the FD method one molecule of β-CD is sufficient to form the complex with NYS. In contrast, the inclusion complex of NYS: β-CD by the SD method is formed by two molecules of β-CD. These results showed the effectiveness of these techniques in evaluating the inclusion complex and demonstrate that the drying method affects how this complexation occurs.
Source: Materials Chemistry and Physics - Category: Materials Science Source Type: research