Solid phase microextraction as a powerful alternative for screening of secondary metabolites in actinomycetes

AbstractActinobacteria are one of the most promising producers of medically and industrially relevant secondary metabolites. However, screening of such compounds in actinobacteria growth demands simple, fast, and efficient extraction procedures that enable detection and precise quantification of biologically active compounds. In this regard, solid phase microextraction (SPME) emerges as an ideal extraction technique for screening of secondary metabolites in bacteria culture due to its non ‐exhaustive, minimally invasive, and non‐destructive nature; its integrated sample preparation workflow; balanced coverage feature; metabolism quenching capabilities; and superior cleanup, as well as its versatility in configuration, which enables automation and high throughput applications. The current work provides a comparison of micro‐scale and direct immersion SPME (DI‐SPME) for screening of secondary metabolites, describes the optimization of the developed DI‐SPME method, and introduces the developed technique for mapping of target secondary metabolites as well as its direct co upling to mass spectrometry for such applications. The optimized DI‐SPME method provided higher amounts of extracted ions and intensity signals, yielding superior extraction and desorption efficiency as compared to micro‐scale extraction. Studied compounds presented stability on the coating for 24 h at room temperature. The DI‐SPME mapping approach revealed that Lysolipin I and the Lienomycin ana...

Source: Journal of Mass Spectrometry - September 1, 2019 Category: Chemistry Authors: Vinicius Ricardo Acquaro Junior, J úlia Pereira Rodrigues, Luiz Alberto Beraldo Moraes Tags: RESEARCH ARTICLE Source Type: research