Immunodiagnostic of leprosy exploiting a photoelectrochemical platform based on a recombinant peptide mimetic of a Mycobacterium leprae antigen

Publication date: 15 October 2019Source: Biosensors and Bioelectronics, Volume 143Author(s): Sakae Yotsumoto Neto, Mayara Ingrid Sousa Lima, Silma Regina Ferreira Pereira, Luiz Ricardo Goulart, Rita de Cássia Silva Luz, Flavio Santos DamosAbstractThe first serum diagnosis of leprosy based on the detection of antibodies of patients using a recombinant mimetic peptide (PGL1M3R) as recognition element and exploiting a photoelectrochemical sensor is presented in this work. The photoeletrochemical platform consists of cadmium sulphide and nickel hydroxide electrodeposited on fluorine-doped tin oxide coated glass slide (CdS/Ni(OH)2/FTO). The optical band gap and flat band potential of the photoelectroactive materials were evaluated by UV–Vis spectroscopy and electrochemical impedance spectroscopy. The spatial photoelectrochemical response of the platform was evaluated by Scanning Electrochemical Microscopy and the morphology of the films was investigated by Scanning Electron Microscopy (SEM). The photoelectrochemical response of the CdS/Ni(OH)2/FTO platform was optimized by evaluating the effects of the kind, concentration, and pH of the buffer. Furthermore, the applied potential to the CdS/Ni(OH)2/FTO platform was also investigated. The CdS/Ni(OH)2/FTO photoelectrochemical platform was modified with a synthetic peptide by using glutaraldehyde as cross-linking reagent and chitosan (CS) for the covalent coupling of the peptide to the photoelectrochemical platform (PGL1M3R/CdS/Ni(...
Source: Biosensors and Bioelectronics - Category: Biotechnology Source Type: research